Institute of Zoology and Center for Molecular Biosciences, University of Innsbruck, Austria.
Cell Tissue Res. 2010 Mar;339(3):527-42. doi: 10.1007/s00441-009-0915-6. Epub 2010 Feb 2.
Stem cells are the only proliferating cells in flatworms and can be eliminated by irradiation with no damage to differentiated cells. We investigated the effect of fractionated irradiation schemes on Macrostomum lignano, namely, on survival, gene expression, morphology and regeneration. Proliferating cells were almost undetectable during the first week post-treatment. Cell proliferation and gene expression were restored within 1 month in a dose-dependent manner following exposure to up to 150 Gy irradiation. During recovery, stem cells did not cross the midline but were restricted within lateral compartments. An accumulated dose of 210 Gy resulted in a lethal phenotype. Our findings demonstrate that M. lignano represents a suitable model system for elucidating the effect of irradiation on the stem cell system in flatworms and for improving our understanding of the recovery potential of severely damaged stem-cell systems.
干细胞是扁形动物中唯一具有增殖能力的细胞，它们可以通过照射被消除，而不会对分化细胞造成损伤。我们研究了分次照射方案对 Macrostomum lignano 的影响，即对存活、基因表达、形态和再生的影响。在治疗后第一周，增殖细胞几乎无法检测到。在暴露于高达 150Gy 的照射下，细胞增殖和基因表达以剂量依赖性方式在 1 个月内得到恢复。在恢复过程中，干细胞没有穿过中线，而是局限在侧腔室中。累积剂量为 210Gy 导致致命表型。我们的研究结果表明，M. lignano 是阐明照射对扁形动物干细胞系统影响的合适模型系统，并且可以提高我们对严重受损干细胞系统的恢复潜力的理解。
