Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.
Chemistry. 2010 Feb 22;16(8):2401-6. doi: 10.1002/chem.200902882.
We have developed an induced circular dichroism (ICD) probe with a chromophore-linked alkynyldeoxyribose skeleton for analyzing higher-order structures of DNA duplexes in the visible-light region. When CG-repeated oligonucleotides (ODNs) with the probe at their 5' ends adopted Z-form duplexes at a high NaCl concentration, strong ICD signals were observed at the absorptive region of the chromophore. On the other hand, their B-form duplexes, formed at a low NaCl concentration, produced a faint ICD signal. The specific ICD for the Z-form duplexes was found to appear only when the chromophores were attached at the 5' ends of each of the ODNs. Furthermore, the chromophoric alkynylnucleoside residues effectively promoted the B to Z transition of the ODN.
我们开发了一种具有发色团连接的炔脱氧核糖骨架的诱导圆二色性（ICD）探针，用于在可见光区域分析 DNA 双链体的高级结构。当在高 NaCl 浓度下探针位于其 5' 端的 CG 重复寡核苷酸（ODN）采用 Z 型双链体时，在发色团的吸收区域观察到强 ICD 信号。另一方面，在低 NaCl 浓度下形成的它们的 B 型双链体产生微弱的 ICD 信号。仅当发色团附着在每个 ODN 的 5' 末端时，才发现 Z 型双链体的特定 ICD。此外，发色团炔基核苷碱基有效地促进了 ODN 的 B 到 Z 的转变。
