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天然植物转基因中的地理和分子变异。

Geographic and molecular variation in a natural plant transgene.

作者信息

Vallenback Pernilla, Bengtsson Bengt O, Ghatnekar Lena

机构信息

Genetics, Department of Cell and Organism Biology, Lund University, Lund, Sweden.

出版信息

Genetica. 2010 Mar;138(3):355-62. doi: 10.1007/s10709-009-9425-4.

DOI:10.1007/s10709-009-9425-4
PMID:20128113
Abstract

A PCR based survey of Festuca ovina plants from populations around the southern part of the Baltic Sea demonstrates both geographic and molecular variation in the enzyme gene PgiC2, horizontally transferred from a Poa-species. Our results show that PgiC2-a natural functional nuclear transgene-is not a local ephemeral phenomenon but is present in a very large number of individuals. We find also that its frequency is geographically variable and that it appears in more than one molecular form. The chloroplast variation in the region does not indicate any distinct subdivision due to different colonization routes after the last glaciation. Our data illustrate the geographic and molecular variation that may occur in natural populations with a polymorphic, unfixed transgene affected by diverse kinds of mutational and evolutionary processes.

摘要

基于聚合酶链式反应(PCR)对波罗的海南部周边种群的羊茅植物进行的调查表明,从早熟禾属物种水平转移而来的酶基因PgiC2存在地理和分子变异。我们的结果表明,PgiC2——一种天然的功能性核转基因——并非局部短暂现象,而是存在于大量个体中。我们还发现其频率存在地理差异,并且呈现出不止一种分子形式。该地区叶绿体的变异并未表明由于末次冰期后不同的定殖路线而产生任何明显的细分。我们的数据说明了在受各种突变和进化过程影响的具有多态性、未固定转基因的自然种群中可能发生的地理和分子变异。

相似文献

1
Geographic and molecular variation in a natural plant transgene.天然植物转基因中的地理和分子变异。
Genetica. 2010 Mar;138(3):355-62. doi: 10.1007/s10709-009-9425-4.
2
Origin and timing of the horizontal transfer of a PgiC gene from Poa to Festuca ovina.PgiC基因从早熟禾向羊茅水平转移的起源与时间。
Mol Phylogenet Evol. 2008 Mar;46(3):890-6. doi: 10.1016/j.ympev.2007.11.031. Epub 2007 Dec 10.
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The introgression of a functional nuclear gene from Poa to Festuca ovina.一个功能性核基因从早熟禾渗入到羊茅属植物羊茅中。
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A horizontally transferred nuclear gene is associated with microhabitat variation in a natural plant population.一个水平转移的核基因与一个天然植物种群中的微生境变异相关。
Proc Biol Sci. 2015 Dec 22;282(1821):20152453. doi: 10.1098/rspb.2015.2453.
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Evidence for Positive Selection within the PgiC1 Locus in the Grass Festuca ovina.羊茅(Festuca ovina)中磷酸葡萄糖异构酶C1(PgiC1)基因座内正选择的证据。
PLoS One. 2015 May 6;10(5):e0125831. doi: 10.1371/journal.pone.0125831. eCollection 2015.
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Contrasting patterns of nucleotide polymorphism suggest different selective regimes within different parts of the gene in L.核苷酸多态性的对比模式表明该基因在L.的不同区域内存在不同的选择机制。
Hereditas. 2017 May 18;154:11. doi: 10.1186/s41065-017-0032-6. eCollection 2017.
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When a foreign gene meets its native counterpart: computational biophysics analysis of two PgiC loci in the grass Festuca ovina.当外来基因遇到其同源物时:对羊茅属植物两个 PgiC 基因座的计算生物物理分析。
Sci Rep. 2020 Oct 30;10(1):18752. doi: 10.1038/s41598-020-75650-0.
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PLoS One. 2010 Oct 20;5(10):e13529. doi: 10.1371/journal.pone.0013529.
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Low variability of internal transcribed spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (POACEAE).羊茅复合体(禾本科)中几个类群的内部转录间隔区rDNA和trnL(UAA)内含子序列的低变异性
Acta Biol Hung. 2006 Mar;57(1):57-69. doi: 10.1556/ABiol.57.2006.1.6.

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Mol Biol Evol. 2021 Aug 23;38(9):3664-3675. doi: 10.1093/molbev/msab133.
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When a foreign gene meets its native counterpart: computational biophysics analysis of two PgiC loci in the grass Festuca ovina.当外来基因遇到其同源物时:对羊茅属植物两个 PgiC 基因座的计算生物物理分析。
Sci Rep. 2020 Oct 30;10(1):18752. doi: 10.1038/s41598-020-75650-0.
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Small spaces, big impacts: contributions of micro-environmental variation to population persistence under climate change.

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Origin and timing of the horizontal transfer of a PgiC gene from Poa to Festuca ovina.PgiC基因从早熟禾向羊茅水平转移的起源与时间。
Mol Phylogenet Evol. 2008 Mar;46(3):890-6. doi: 10.1016/j.ympev.2007.11.031. Epub 2007 Dec 10.
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Hereditas. 2017 May 18;154:11. doi: 10.1186/s41065-017-0032-6. eCollection 2017.
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A horizontally transferred nuclear gene is associated with microhabitat variation in a natural plant population.一个水平转移的核基因与一个天然植物种群中的微生境变异相关。
Proc Biol Sci. 2015 Dec 22;282(1821):20152453. doi: 10.1098/rspb.2015.2453.
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Evidence for Positive Selection within the PgiC1 Locus in the Grass Festuca ovina.羊茅(Festuca ovina)中磷酸葡萄糖异构酶C1(PgiC1)基因座内正选择的证据。
PLoS One. 2015 May 6;10(5):e0125831. doi: 10.1371/journal.pone.0125831. eCollection 2015.
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Structure of the natural transgene PgiC2 in the common grass Festuca ovina.天然转基因 PgiC2 在普通羊茅属植物中的结构。
PLoS One. 2010 Oct 20;5(10):e13529. doi: 10.1371/journal.pone.0013529.
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Horizontal gene transfer in plants.植物中的水平基因转移
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The introgression of a functional nuclear gene from Poa to Festuca ovina.一个功能性核基因从早熟禾渗入到羊茅属植物羊茅中。
Proc Biol Sci. 2006 Feb 22;273(1585):395-9. doi: 10.1098/rspb.2005.3355.
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Pseudogenes: are they "junk" or functional DNA?假基因:它们是“垃圾”还是功能性DNA?
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A DNA marker for the duplicated cytosolic PGI genes in sheep's fescue (Festuca ovina L.).羊茅(Festuca ovina L.)中重复的胞质磷酸葡萄糖异构酶基因的DNA标记。
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A polymorphic duplicated locus for cytosolic PGI segregating in sheep's fescue (Festuca ovina L.).一个在羊茅(Festuca ovina L.)中分离的胞质磷酸葡萄糖异构酶多态性重复位点。
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