[头皮针疗法对急性脑缺血再灌注损伤大鼠核因子κB信使核糖核酸、环氧化酶-2信使核糖核酸及其蛋白表达的影响]

[Effect of scalp acupuncture on the expression of NF-kappaB mRNA, COX-2 mRNA and their proteins in rats with acute cerebral ischemia-reperfusion injury].

作者信息

Zhou Li, Zhang Hong-Xing, Wang Qiong, Liu Ling-Guang, Yang Xuan, Yang Min, Liu Yin-Ni, Li Xuan

机构信息

Department of Acu-morxibustion, Wuhan Hospital of Integrated Chinese & Western Medicine, Wuhan 430022, China.

出版信息

Zhen Ci Yan Jiu. 2009 Oct;34(5):304-8.

PMID:20128288

Abstract

OBJECTIVE

To explore the possible mechanism of scalp acupuncture (SA) in relieving cerebral ischemia reperfusion (CI-R) injury.

METHODS

Seventy male SD rats were randomized into sham-operation (sham, n = 10), model (n = 30) and SA (n = 30) groups. The later 2 groups were further divided into 24 h, 48 h and 72 h subgroups respectively, with 10 cases in each. CI-R model was established by middle cerebral artery occlusion (MCAO) and reperfusion. Electroacupuncture (2 mA, 2 Hz/100 Hz) was applied to "Dingnie Houxiexian" (MS 7) and "Dingnie Qianxiexian" (MS 6) for 30 min, once every 24 h. Changes of the animal behavior were observed by using neurological severity score (NSS), nuclear factor-kappa B (NF-kappaB) and cyclooxygenase (COX-2) contents and their mRNA expression were detected with enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) techniques respectively.

RESULTS

Following modeling, the NSS at 24 h, 48 h and 72 h after MCAO increased significantly, while compared with model group, NSS of SA group at the 3 time-points decreased considerably (P < 0.05, P < 0.01), suggesting an improvement of the neurological functions after SA treatment. In comparison with sham group, NF-kappaB mRNA and COX-2 mRNA, and NF-kappaB and COX-2 protein expression of model group in the infarcted cerebral tissue were significantly upregulated at the 3 time-points (P < 0.01) except NF-KB mRNA at 72 h (no significant change), while compared with the 3 time-points of model group, NF-kappaB mRNA and COX-2 mRNA, and NF-KB and COX-2 protein expression of SA group were downregulated obviously (P < 0.01) except COX-2 mRNA at 72 h.

CONCLUSION

Scalp acupuncture can suppress cerebral ischemia-induced upregulation of NF-kappaB mRNA and COX-2 mRNA, and NF-kappaB and COX-2 protein expression, which may contribute to its effect in promoting neurofunctional rehabilitation of CI-R rats by reducing cytokines-mediated inflammatory reaction.

摘要

目的

探讨头皮针缓解脑缺血再灌注（CI-R）损伤的可能机制。

方法

将70只雄性SD大鼠随机分为假手术组（假手术组，n = 10）、模型组（n = 30）和头皮针组（n = 30）。后两组再分别分为24 h、48 h和72 h亚组，每组10只。采用大脑中动脉闭塞（MCAO）再灌注法建立CI-R模型。电针（2 mA，2 Hz/100 Hz）刺激“顶颞后斜线”（MS 7）和“顶颞前斜线”（MS 6）30分钟，每24小时1次。采用神经功能缺损评分（NSS）观察动物行为变化，分别采用酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）技术检测核因子-κB（NF-κB）和环氧化酶（COX-2）含量及其mRNA表达。

结果

建模后，MCAO后24 h、48 h和72 h的NSS显著升高，而与模型组相比，头皮针组在这3个时间点的NSS显著降低（P < 0.05，P < 0.01），提示头皮针治疗后神经功能得到改善。与假手术组相比，模型组梗死脑组织在3个时间点的NF-κB mRNA和COX-2 mRNA以及NF-κB和COX-2蛋白表达均显著上调（P < 0.01），72 h时NF-KB mRNA除外（无显著变化），而与模型组的3个时间点相比，头皮针组的NF-κB mRNA和COX-2 mRNA以及NF-KB和COX-2蛋白表达均明显下调（P < 0.01），72 h时COX-2 mRNA除外。