Lavalett Lelia, Sánchez Miryan Margot, Múñoz Nélida, Moreno Jaime, Cardona-Castro Nora
Universidad Nacional de Colombia, Medellín, Colombia.
Biomedica. 2009 Jun;29(2):244-52.
The scheme Kauffman-White (KW) for serotyping of Salmonella recognizes 46 O antigens, and 119 H antigens, thereby permitting the characterization of 2541 serotypes. The serotyping is a useful epidemiological tool in identifying circulating serotypes and to characterize outbreaks. However, the method presents technical limitations, difficulty in interpretation of results and high costs.
A multiplex polymerase chain reaction test (M-PCR) was developed as an alternative method for the identification of serogroups B, C2, D, and E of Salmonella enterica.
The M-PCR detected Salmonella genes rfbJ of serogroups B and C2 and wzx of serogroups D and E. To standardize the M-PCR, reference strains of Salmonella serogroups were compared. Amplification of invA gender-specific gene of Salmonella was included as internal control of amplification. To validate the test, a blind study was conducted to identify by M-PCR 400 isolates that had been previously characterized by serology.
The M-PCR detected Salmonella serogroups with reproducible results (Kappa index = 0.95). The sensitivity of the test was between 98% to 100% and specificity between 96% to 100%.
The polymorphisms in the Salmonella genes rfbJ and wzx permitted the development of a method for molecular typing of Salmonella serogroups that was sensitive, specific and reproducible.
用于沙门氏菌血清分型的考夫曼-怀特（KW）方案识别46种O抗原和119种H抗原，从而能够鉴定出2541种血清型。血清分型是一种有用的流行病学工具，可用于识别流行的血清型并对疫情进行特征描述。然而，该方法存在技术局限性、结果解读困难以及成本高昂的问题。
开发一种多重聚合酶链反应试验（M-PCR）作为鉴定肠炎沙门氏菌B、C2、D和E血清群的替代方法。
M-PCR检测血清群B和C2的沙门氏菌基因rfbJ以及血清群D和E的wzx。为使M-PCR标准化，对沙门氏菌血清群的参考菌株进行了比较。将沙门氏菌invA性别特异性基因的扩增作为扩增的内部对照。为验证该试验，进行了一项盲法研究，以通过M-PCR鉴定400株先前已通过血清学鉴定的分离株。
M-PCR检测沙门氏菌血清群的结果具有可重复性（卡帕指数=0.95）。该试验的灵敏度在98%至100%之间，特异性在96%至100%之间。
沙门氏菌基因rfbJ和wzx中的多态性使得能够开发出一种用于沙门氏菌血清群分子分型的方法，该方法灵敏、特异且可重复。
