5-脂氧合酶途径基因多态性：与西班牙人群哮喘无关。

5-Lipoxygenase pathway gene polymorphisms: lack of association with asthma in a Spanish population.

机构信息

Research Unit, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Spain.

出版信息

J Investig Allergol Clin Immunol. 2009;19(6):453-8.

PMID:20128419

Abstract

BACKGROUND

Cysteinyl-leukotrienes are mediators of inflammatory responses in bronchial asthma. We studied the genes encoding the enzymes involved in their synthesis to identify risk factors for asthma. The promoter polymorphisms LTC4S -444 A/C, ALOX5 -176/-147, and ALOX5AP -169/-146 have been reported to be associated with bronchial asthma.

METHODS

We analyzed the effects of LTC4S -444 A/C, ALOX5 -176/-147, and ALOX5AP -169/-146 on asthma susceptibility by means of a case-control study with 193 ethnically matched, unrelated individuals. Participants were classified as severe asthmatics, nonsevere asthmatics, and nonasthmatics, using a combination of 2 techniques: polymerase chain reaction-restricted fragment length polymorphism and multiplex capillary electrophoresis.

RESULTS

No association was found between these polymorphisms and asthma, neither individually nor in combination.

CONCLUSION

Although the studied polymorphisms have been previously reported to constitute risk factors for the disease, we found no association between LTC4S -444 A/C, ALOX5 -176/-147, and ALOX5AP -169/-146 polymorphisms and bronchial asthma.

摘要

背景

半胱氨酰白三烯是支气管哮喘炎症反应的介质。我们研究了参与其合成的酶的基因，以确定哮喘的危险因素。先前已经报道 LTC4S-444A/C、ALOX5-176/-147 和 ALOX5AP-169/-146 启动子多态性与支气管哮喘有关。

方法

我们通过一项包含 193 名种族匹配、无关个体的病例对照研究，分析了 LTC4S-444A/C、ALOX5-176/-147 和 ALOX5AP-169/-146 对哮喘易感性的影响。参与者通过聚合酶链反应-限制性片段长度多态性和多重毛细管电泳相结合的技术分为严重哮喘患者、非严重哮喘患者和非哮喘患者。