Orlu Eme E, Egbunike G N
Department of Applied and Environmental Biology, Rivers State University of Science and Technology, P.M.B. 5080, Nkpolu, Port Harcourt, Rivers State, Nigeria.
Pak J Biol Sci. 2009 Oct 15;12(20):1359-64. doi: 10.3923/pjbs.2009.1359.1364.
The daily spermatozoa production was studied in 20 each of sexually matured barred Plymouth Rock and Nigerian indigenous breeds of domestic fowl using both the histometric and testicular homogenate methods. The exotic cocks were significantly (p < 0.01) heavier than the locals with the respective values of 2.11 +/- 0.05 and 1.58 +/- 0.02 kg. The exotic also had larger (p < 0.01) gross testicular weight (21.58 +/- 1.46 vs. 12.56 +/- 0.91 g), paired testicular parenchymal weight (20.47 +/- 0.40 vs. 11.96 +/- 0.82 g) and paired tunica albuginea (1.11 +/- 0.18 vs. 0.60 +/- 0.11 g). The total length and the diameter of the seminiferous tubules were also significantly influenced (p < 0.01) by breed. The volume percent occupied by seminiferous tubules though higher in the exotic birds showed no statistical significance. Daily sperm production obtained from histometric method was highly influenced by breed with 2.41 +/- 1.17 x 10(9) and 0.76 +/- 0.71 x 10(9) for exotic and local cocks, respectively. The daily sperm productions calculated on the basis of homogenization-resistant spermatids were 1.85 +/- 0.22 for exotic and 0.73 +/- 0.11 for locals. Although, the difference in DSP values based on both methods was not statistically significant there was a 23.24 and 3.90% loss in exotic and local birds respectively with the use of homogenization method. On the whole, the exotic cocks were twice as efficient in sperm production as the local birds.
采用组织测量法和睾丸匀浆法，对20只性成熟的横斑洛克鸡和20只尼日利亚本土家鸡的每日精子生成量进行了研究。外来品种公鸡体重显著高于本地品种公鸡（p < 0.01），分别为2.11±0.05千克和1.58±0.02千克。外来品种公鸡的总睾丸重量（21.58±1.46克对12.56±0.91克）、双侧睾丸实质重量（20.47±0.40克对11.96±0.82克）和双侧白膜重量（1.11±0.18克对0.60±0.11克）也显著更大（p < 0.01）。品种对曲细精管的总长度和直径也有显著影响（p < 0.01）。曲细精管所占的体积百分比在外来品种鸡中虽较高，但无统计学意义。通过组织测量法得到的每日精子生成量受品种影响很大，外来品种公鸡和本地品种公鸡分别为2.41±1.17×10⁹和0.76±0.71×10⁹。基于抗匀浆精子细胞计算的外来品种公鸡和本地品种公鸡的每日精子生成量分别为1.85±0.22和0.73±0.11。虽然基于两种方法的每日精子生成量差值无统计学意义，但使用匀浆法时，外来品种鸡和本地品种鸡分别损失了23.24%和3.90%。总体而言，外来品种公鸡的精子生成效率是本地品种公鸡的两倍。
