二维高效薄层层析部分分离后解吸电喷雾电离质谱分析脂质。
Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation.
机构信息
Department of Biomedical Sciences, Faculty of Medicine, University of Foggia, Viale L. Pinto, 1-71100 Foggia, Italy.
出版信息
Anal Chem. 2010 Mar 1;82(5):1744-50. doi: 10.1021/ac902325j.
Abstract
Molecular imaging of separate but still incompletely resolved spots on high-performance thin-layer chromatography (HPTLC) plates is used for the direct analysis of porcine brain lipids by desorption electrospray ionization mass spectrometry (DESI-MS). Eight class-specific spots were imaged in the negative ion mode and shown to contain more than fifty lipids. A low lateral resolution of 400 x 400 microm allowed simple, rapid, and incomplete separation to be combined with DESI imaging for the identification of many components of these extremely complex mixtures. In this work, tandem mass spectrometry (MS/MS) was also employed to confirm the identity of particular lipids directly on HPTLC plates.
摘要
采用高效薄层色谱（HPTLC）板上独立但仍未完全解析的斑点的分子成像，通过解吸电喷雾电离质谱（DESI-MS）直接分析猪脑脂质。在负离子模式下对 8 个类特异性斑点进行成像，结果表明这些斑点含有五十多种脂质。较低的横向分辨率为 400 x 400 微米，这允许简单、快速和不完全的分离与 DESI 成像相结合，以鉴定这些极其复杂混合物的许多成分。在这项工作中，串联质谱（MS/MS）也被用于直接在 HPTLC 板上确认特定脂质的身份。
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