• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

二维高效薄层层析部分分离后解吸电喷雾电离质谱分析脂质。

Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation.

机构信息

Department of Biomedical Sciences, Faculty of Medicine, University of Foggia, Viale L. Pinto, 1-71100 Foggia, Italy.

出版信息

Anal Chem. 2010 Mar 1;82(5):1744-50. doi: 10.1021/ac902325j.

DOI:10.1021/ac902325j
PMID:20128616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2830312/
Abstract

Molecular imaging of separate but still incompletely resolved spots on high-performance thin-layer chromatography (HPTLC) plates is used for the direct analysis of porcine brain lipids by desorption electrospray ionization mass spectrometry (DESI-MS). Eight class-specific spots were imaged in the negative ion mode and shown to contain more than fifty lipids. A low lateral resolution of 400 x 400 microm allowed simple, rapid, and incomplete separation to be combined with DESI imaging for the identification of many components of these extremely complex mixtures. In this work, tandem mass spectrometry (MS/MS) was also employed to confirm the identity of particular lipids directly on HPTLC plates.

摘要

采用高效薄层色谱（HPTLC）板上独立但仍未完全解析的斑点的分子成像，通过解吸电喷雾电离质谱（DESI-MS）直接分析猪脑脂质。在负离子模式下对 8 个类特异性斑点进行成像，结果表明这些斑点含有五十多种脂质。较低的横向分辨率为 400 x 400 微米，这允许简单、快速和不完全的分离与 DESI 成像相结合，以鉴定这些极其复杂混合物的许多成分。在这项工作中，串联质谱（MS/MS）也被用于直接在 HPTLC 板上确认特定脂质的身份。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/dcb7e7ea9ba1/nihms176750f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/72e59b2bad1f/nihms176750f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/96e752edc7c7/nihms176750f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/27b7be5dd37f/nihms176750f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/550407c5181b/nihms176750f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/dcb7e7ea9ba1/nihms176750f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/72e59b2bad1f/nihms176750f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/96e752edc7c7/nihms176750f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/27b7be5dd37f/nihms176750f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/550407c5181b/nihms176750f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3e6f/2830312/dcb7e7ea9ba1/nihms176750f5.jpg

相似文献

1
Desorption electrospray ionization mass spectrometry analysis of lipids after two-dimensional high-performance thin-layer chromatography partial separation.二维高效薄层层析部分分离后解吸电喷雾电离质谱分析脂质。
Anal Chem. 2010 Mar 1;82(5):1744-50. doi: 10.1021/ac902325j.
2
Elution, partial separation, and identification of lipids directly from tissue slices on planar chromatography media by desorption electrospray ionization mass spectrometry.通过解吸电喷雾电离质谱法直接从平面色谱介质上的组织切片中洗脱、部分分离和鉴定脂质。
Anal Chem. 2010 Nov 1;82(21):8866-74. doi: 10.1021/ac1016453. Epub 2010 Oct 13.
3
Identification of Sassafras albidum alkaloids by high-performance thin-layer chromatography tandem mass spectrometry and mapping by desorption electrospray ionization mass spectrometry imaging.采用高效薄层色谱串联质谱法鉴定白木薫堿类，并通过解吸电喷雾电离质谱成像进行定位。
J Mass Spectrom. 2021 Jan;56(1):e4674. doi: 10.1002/jms.4674. Epub 2020 Nov 6.
4
Analysis of human plasma lipids and soybean lecithin by means of high-performance thin-layer chromatography and matrix-assisted laser desorption/ionization mass spectrometry.利用高效薄层色谱法和基质辅助激光解吸/电离质谱法分析人血浆脂质和大豆卵磷脂。
Rapid Commun Mass Spectrom. 2009 Sep;23(17):2711-23. doi: 10.1002/rcm.4173.
5
Direct analysis of Salvia divinorum leaves for salvinorin A by thin layer chromatography and desorption electrospray ionization multi-stage tandem mass spectrometry.薄层层析-解吸电喷雾串联质谱法直接分析迷幻鼠尾草叶片中的蟾毒色胺 A
Rapid Commun Mass Spectrom. 2010 May 15;24(9):1305-11. doi: 10.1002/rcm.4514.
6
High performance Reversed-Phase Thin-Layer Chromatography-Desorption electrospray ionisation - time of flight high resolution mass spectrometric detection and imaging (HPTLC/DESI/ToFMS) of phytoecdysteroids.高效反相薄层层析-解吸电喷雾离子化-飞行时间高分辨质谱检测和成像（HPTLC/DESI/ToFMS）法测定植物蜕皮甾酮。
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Jun 1;1200:123265. doi: 10.1016/j.jchromb.2022.123265. Epub 2022 Apr 27.
7
High-performance thin-layer chromatography/desorption electrospray ionization mass spectrometry imaging of the crude extract from the peels of Citrus aurantium L. (Rutaceae).酸橙（芸香科）果皮粗提物的高效薄层色谱/解吸电喷雾电离质谱成像
Rapid Commun Mass Spectrom. 2015 Aug 30;29(16):1530-4. doi: 10.1002/rcm.7246.
8
Direct analysis of pharmaceutical formulations from non-bonded reversed-phase thin-layer chromatography plates by desorption electrospray ionisation ion mobility mass spectrometry.通过解吸电喷雾电离离子淌度质谱法对非键合反相薄层色谱板上的药物制剂进行直接分析。
Rapid Commun Mass Spectrom. 2009 Sep;23(17):2597-604. doi: 10.1002/rcm.4152.
9
Chemical profiling and separation of bioactive secondary metabolites in Maca (Lepidium peruvianum) by normal and reverse phase thin layer chromatography coupled to desorption electrospray ionization-mass spectrometry.通过正相和反相薄层色谱结合解吸电喷雾电离质谱法对玛咖（秘鲁独行菜）中生物活性次生代谢产物进行化学分析和分离
J Mass Spectrom. 2021 Feb;56(2):e4690. doi: 10.1002/jms.4690.
10
Monolithic superhydrophobic polymer layer with photopatterned virtual channel for the separation of peptides using two-dimensional thin layer chromatography-desorption electrospray ionization mass spectrometry.采用二维薄层色谱-解吸电喷雾电离质谱法，通过光图案虚拟通道对肽进行分离的整体超疏水聚合物层。
Anal Chem. 2010 Mar 15;82(6):2520-8. doi: 10.1021/ac100010h.

引用本文的文献

1
Applications of chromatographic methods in metabolomics: A review.色谱法在代谢组学中的应用：综述。
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 May 15;1239:124124. doi: 10.1016/j.jchromb.2024.124124. Epub 2024 Apr 16.
2
Quantitative detection of caffeine in beverages using flowing atmospheric-pressure afterglow (FAPA) ionization high-resolution mass spectrometry imaging and performance evaluation of different thin-layer chromatography plates as sample substrates.利用流动常压余晖（FAPA）电离高分辨质谱成像技术对饮料中的咖啡因进行定量检测及不同薄层色谱板作为样品基质的性能评价。
Anal Bioanal Chem. 2022 Jun;414(15):4481-4495. doi: 10.1007/s00216-022-04045-z. Epub 2022 Apr 20.
3

本文引用的文献

1
Analysis of biodiesel and biodiesel-petrodiesel blends by high performance thin layer chromatography combined with easy ambient sonic-spray ionization mass spectrometry.采用高效薄层色谱法与简易环境声喷雾电离质谱联用技术分析生物柴油和生物柴油-石油柴油混合物。
Analyst. 2009 Aug;134(8):1652-7. doi: 10.1039/b817847j. Epub 2009 Jun 26.
2
Lipid profiles of canine invasive transitional cell carcinoma of the urinary bladder and adjacent normal tissue by desorption electrospray ionization imaging mass spectrometry.采用解吸电喷雾成像质谱法分析犬浸润性移行细胞膀胱癌及其邻近正常组织的脂质图谱。
Anal Chem. 2009 Nov 1;81(21):8758-64. doi: 10.1021/ac901028b.
3
HPTLC and FTIR Fingerprinting of Olive Leaves Extracts and ATR-FTIR Characterisation of Major Flavonoids and Polyphenolics.
HPTLC 和 FTIR 橄榄油叶提取物指纹图谱分析及主要类黄酮和多酚的 ATR-FTIR 特性研究。
Molecules. 2021 Nov 16;26(22):6892. doi: 10.3390/molecules26226892.
4
Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization.结合使用簇诱导解吸/电离的薄层色谱法和质谱分析法
ACS Omega. 2019 Dec 20;4(27):22426-22430. doi: 10.1021/acsomega.9b03060. eCollection 2019 Dec 31.
5
Lipid Coverage in Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Mouse Lung Tissues.纳米喷雾解吸电喷雾电离质谱成像技术分析小鼠肺组织中的脂质覆盖情况。
Anal Chem. 2019 Sep 17;91(18):11629-11635. doi: 10.1021/acs.analchem.9b02045. Epub 2019 Aug 27.
6
Addition of Serine Enhances Protein Analysis by DESI-MS.丝氨酸的添加增强了 DESI-MS 的蛋白质分析。
J Am Soc Mass Spectrom. 2019 Apr;30(4):694-703. doi: 10.1007/s13361-018-02129-8. Epub 2019 Feb 15.
7
Molecular typing of Meningiomas by Desorption Electrospray Ionization Mass Spectrometry Imaging for Surgical Decision-Making.通过解吸电喷雾电离质谱成像对脑膜瘤进行分子分型以辅助手术决策
Int J Mass Spectrom. 2015 Feb 1;377:690-698. doi: 10.1016/j.ijms.2014.06.024.
8
GRAF1a is a brain-specific protein that promotes lipid droplet clustering and growth, and is enriched at lipid droplet junctions.GRAF1a是一种大脑特异性蛋白，可促进脂滴聚集和生长，并在脂滴连接处富集。
J Cell Sci. 2014 Nov 1;127(Pt 21):4602-19. doi: 10.1242/jcs.147694. Epub 2014 Sep 4.
9
Transmission geometry laserspray ionization vacuum using an atmospheric pressure inlet.采用大气压入口的传输几何结构激光喷雾电离真空系统。
Anal Chem. 2014 Jul 1;86(13):6208-13. doi: 10.1021/ac501788p. Epub 2014 Jun 18.
10
Ion mobility derived collision cross sections to support metabolomics applications.用于支持代谢组学应用的离子淌度衍生碰撞截面
Anal Chem. 2014 Apr 15;86(8):3985-93. doi: 10.1021/ac500405x. Epub 2014 Mar 28.
Rapid, direct analysis of cholesterol by charge labeling in reactive desorption electrospray ionization.
通过反应性解吸电喷雾电离中的电荷标记对胆固醇进行快速、直接分析。
Anal Chem. 2009 Sep 15;81(18):7618-24. doi: 10.1021/ac901003u.
4
Direct coupling of high-performance thin-layer chromatography with UV spectroscopy and IR-MALDI orthogonal TOF MS for the analysis of cyanobacterial toxins.高效薄层色谱与紫外光谱及红外基质辅助激光解吸电离正交飞行时间质谱直接联用用于蓝藻毒素分析
Anal Chem. 2009 May 15;81(10):3858-66. doi: 10.1021/ac900217q.
5
Sphingolipidomics: a valuable tool for understanding the roles of sphingolipids in biology and disease.鞘脂组学：一种理解鞘脂在生物学和疾病中作用的宝贵工具。
J Lipid Res. 2009 Apr;50 Suppl(Suppl):S97-102. doi: 10.1194/jlr.R800073-JLR200. Epub 2008 Nov 21.
6
High-throughput quantitative analysis by desorption electrospray ionization mass spectrometry.通过解吸电喷雾电离质谱法进行高通量定量分析。
J Am Soc Mass Spectrom. 2009 Feb;20(2):321-5. doi: 10.1016/j.jasms.2008.10.011. Epub 2008 Nov 1.
7
Sterol profiling in red blood cell membranes and plasma of newborns receiving total parenteral nutrition.
J Pediatr Gastroenterol Nutr. 2008 Nov;47(5):645-51. doi: 10.1097/MPG.0b013e318170956a.
8
Recent developments in ambient ionization techniques for analytical mass spectrometry.用于分析质谱的常压电离技术的最新进展。
Analyst. 2008 Oct;133(10):1297-301. doi: 10.1039/b806810k. Epub 2008 Jul 10.
9
Analysis of stem cell lipids by offline HPTLC-MALDI-TOF MS.通过离线高效薄层层析-基质辅助激光解吸电离飞行时间质谱分析干细胞脂质。
Anal Bioanal Chem. 2008 Nov;392(5):849-60. doi: 10.1007/s00216-008-2301-8. Epub 2008 Aug 5.
10
Established and emerging atmospheric pressure surface sampling/ionization techniques for mass spectrometry.用于质谱分析的成熟和新兴的大气压表面采样/电离技术。
J Mass Spectrom. 2008 Sep;43(9):1161-80. doi: 10.1002/jms.1440.