Department of Public Health & Preventive Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Immunopharmacol Immunotoxicol. 2010 Jun;32(2):246-50. doi: 10.3109/08923970903229053.
To determine how estrogen exacerbates allergies, the effects of 17beta-estradiol (E2) on lymphocyte proliferation were investigated. BALB/c mice were ovariectomized, administered 3.2 microg E2, and sensitized with 50 microL 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one. After 7 days, their spleens were excised and flow cytometrically analyzed. The CD8(+)CD45RA(-)CCR7(-) cell-to-CD8(+) cell ratio in the spleen was greater in the E2-administered mice than in the controls. Splenocytes were cultured under concanavalin A stimulation, with or without E2. After 4 days, the above ratio was greater in the case of E2-treated splenocytes. E2 increases the number of effector memory CD8(+) lymphocytes during sensitization in contact hypersensitivity.
为了确定雌激素如何加重过敏反应，研究了 17β-雌二醇（E2）对淋巴细胞增殖的影响。BALB/c 小鼠被卵巢切除术，给予 3.2 微克 E2，并通过 50 微升 4-乙氧亚甲基-2-苯基-2-恶唑啉-5-酮致敏。7 天后，切除其脾脏并进行流式细胞术分析。在给予 E2 的小鼠中，脾脏中 CD8+CD45RA（-）CCR7（-）细胞与 CD8+细胞的比例高于对照组。在刀豆蛋白 A 刺激下培养脾细胞，有或没有 E2。4 天后，E2 处理的脾细胞中上述比例更高。E2 在接触超敏反应的致敏过程中增加效应记忆 CD8+淋巴细胞的数量。
