Pandey Alok, Gupta S C, Gupta Neelam
Transgenic Research Laboratory, DNA Finger Printing Unit, National Bureau of Animal Genetic Resources, Karnal 132001, India.
Zygote. 2010 Aug;18(3):231-4. doi: 10.1017/S096719940999030X. Epub 2010 Feb 4.
Follicle stimulating hormone (FSH) and luteinizing hormone (LH) are commonly added to maturation media to improve cumulus expansion known to be a predictor of oocyte maturation. Therefore, effects of various concentrations of FSH (1000 ng/ml), LH (1000 ng/ml) and FSH + LH (1000 ng/ml each) in comparison with control (without FSH + LH) cultured oocytes were investigated. FSH and LH (1000 ng/ml each) induced significantly more cumulus expansion and polar body numbers, as compared with control and treatments of 1000 ng/ml FSH and 1000 ng/ml LH alone. Expression of FSH receptor (r), LHr and Cx43 mRNAs was determined by real-time PCR in cumulus-oocyte complexes (COCs) and denuded oocytes at different maturation times. Expression of all three genes was higher in COCs compared with denuded oocytes, confirming the importance of cumulus cells in oocyte maturation. FSHr and connexin 43 (Cx43) mRNA abundance in both COCs and denuded oocytes was highest at 0-6 h of maturation and decreased subsequently. However, LHr mRNA abundance increased from 6 h up to 24 h of maturation. The study concluded that FSH, LH receptors and Cx43 gene expression regulation is an index related to oocyte maturation.
促卵泡激素（FSH）和促黄体生成素（LH）通常被添加到成熟培养液中，以促进卵丘扩展，而卵丘扩展是卵母细胞成熟的一个预测指标。因此，本研究调查了不同浓度的FSH（1000 ng/ml）、LH（1000 ng/ml）以及FSH + LH（各1000 ng/ml）与未添加FSH + LH的对照组相比，对培养的卵母细胞的影响。与对照组以及单独使用1000 ng/ml FSH和1000 ng/ml LH的处理组相比，FSH和LH（各1000 ng/ml）显著诱导了更多的卵丘扩展和极体数量。通过实时PCR测定了不同成熟时间的卵丘-卵母细胞复合体（COCs）和裸卵中FSH受体（r）、LH受体（LHr）和Cx43 mRNA的表达。与裸卵相比，所有这三个基因在COCs中的表达更高，这证实了卵丘细胞在卵母细胞成熟中的重要性。COCs和裸卵中FSHr和连接蛋白43（Cx43）mRNA丰度在成熟0 - 6小时时最高，随后下降。然而，LHr mRNA丰度在成熟6小时至24小时期间增加。该研究得出结论，FSH、LH受体和Cx43基因表达调控是与卵母细胞成熟相关的一个指标。
