[黄芪汤抑制胆管结扎诱导的大鼠胆汁淤积性肝纤维化中胆管细胞的增殖和转分化]

[Huangqi decoction inhibits cholangiocyte proliferation and transdifferentiation in cholestatic liver fibrosis induced by BDL in rats].

作者信息

DU Jin-Xing, Qiu Bing-Feng, Liu Ping, Sun Ming-Yu, Chen Gao-Feng, Liu Jia

机构信息

Key Laboratory of Liver and Kidney Diseases, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2010 Jan;18(1):13-8. doi: 10.3760/cma.j.issn.1007-3418.2010.01.004.

DOI:10.3760/cma.j.issn.1007-3418.2010.01.004

PMID:20128962

Abstract

OBJECTIVE

To elucidate the antifibrotic mechanism of Huangqi decoction in rats with BDL-induced cholestatic liver fibrosis.

METHODS

Liver fibrosis model was induced by ligating the common bile duct (BDL) in rats. Sham-operation was performed in control rats. The BDL rats were randomly divided into two groups: the BDL group and the Huangqi decoction group. Huanqi decoction was given intragastrically for 4 weeks. At the end of the fifth week after BDL, animals were sacrificed.

RESULTS

Compared with the sham control group, mortality rate in BDL group was 33.3% and incidence rate of ascites was 90%, and hepatic function was abnormal in most of the rats in BDL group. The number of Hepatocytes was decreased and the number of cholangiocytes significantly increased in BDL group. In addition, Hyp content of liver tissue and protein expression of CK 7 and a-SMA were significantly increased. Immunostaining indicated that CK 7 and a-SMA were co-localized in BDL group. These changes were markedly suppressed by the Huangqi decoction.

CONCLUSIONS

These observations suggest that Huangqi decoction can inhibit cholangiocyte proliferation and cholangiocyte transdifferentiation.

摘要

目的

阐明黄芪汤对胆管结扎（BDL）诱导的大鼠胆汁淤积性肝纤维化的抗纤维化机制。

方法

通过结扎大鼠胆总管（BDL）诱导肝纤维化模型。对对照大鼠进行假手术。将BDL大鼠随机分为两组：BDL组和黄芪汤组。给予黄芪汤灌胃4周。在BDL后第5周结束时，处死动物。

结果

与假手术对照组相比，BDL组死亡率为33.3%，腹水发生率为90%，且BDL组大多数大鼠肝功能异常。BDL组肝细胞数量减少，胆管细胞数量显著增加。此外，肝组织羟脯氨酸（Hyp）含量以及细胞角蛋白7（CK 7）和α-平滑肌肌动蛋白（α-SMA）的蛋白表达显著增加。免疫染色表明，BDL组CK 7和α-SMA共定位。这些变化被黄芪汤显著抑制。