Xiao Zhong, Yi Yong-Fen, He Ting-Ting, Li Yan-Qing
Department of Pathology, Chongqing Medical University, Chongqing 400016, China.
Zhonghua Gan Zang Bing Za Zhi. 2010 Jan;18(1):23-6. doi: 10.3760/cma.j.issn.1007-3418.2010.01.006.
To compare the Golgi proteome of hepatocellular carcinoma (HCC) with that of the adjacent non-tumor tissues.
Hepatocellular carcinoma and adjacent non-tumor tissues were obtained from HCC patients. The protein expression maps in Golgi were obtained by two-dimensional gel electrophoresis (2-DE), and the differentially expressed protein spots were analyzed by PD-Quest software. Peptide mass fingerprint (PMF) of differential protein spots was obtained with MALD-TOT-MS.
According to 2-DE maps, the average numbers of protein spots were (1153+/-49) and (1086+/-37) in hepatocellular carcinoma and the adjacent non-tumor tissues. Compared to the adjacent non-tumor tissues, 27 proteins were upregulated, and 20 proteins were downregulated in HCC Golgi.
The Golgi proteome in HCC tissues is different from that in the adjacent non-tumor tissues, and the differential expression proteins are involved in energy metabolism, tumor metastasis, and cell cycle regulation.
比较肝细胞癌（HCC）与癌旁非肿瘤组织的高尔基体蛋白质组。
从肝癌患者获取肝细胞癌及癌旁非肿瘤组织。通过二维凝胶电泳（2-DE）获得高尔基体中的蛋白质表达图谱，并用PD-Quest软件分析差异表达的蛋白点。用基质辅助激光解吸电离飞行时间质谱（MALD-TOT-MS）获得差异蛋白点的肽质量指纹（PMF）。
根据2-DE图谱，肝细胞癌组织和癌旁非肿瘤组织中蛋白点的平均数分别为（1153±49）和（1086±37）。与癌旁非肿瘤组织相比，肝癌高尔基体中有27种蛋白上调，20种蛋白下调。
肝癌组织中的高尔基体蛋白质组与癌旁非肿瘤组织不同，差异表达的蛋白参与能量代谢、肿瘤转移和细胞周期调控。
