在大肠杆菌中表达的米曲霉糖苷水解酶家族 43 木糖苷酶的特性。
Characterization of Aspergillus oryzae glycoside hydrolase family 43 beta-xylosidase expressed in Escherichia coli.
机构信息
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, Japan.
出版信息
J Biosci Bioeng. 2010 Feb;109(2):115-7. doi: 10.1016/j.jbiosc.2009.07.018. Epub 2009 Sep 12.
PMID:20129093
Abstract
This is the first report of glycoside hydrolase family 43 beta-xylosidase from Aspergillus oryzae. To characterize this enzyme, the recombinant enzyme was expressed in Escherichia coli. Unlike known beta-xylosidases from fungal origins, the enzyme did not show substrate ambiguity and was stable at alkaline pH.
摘要
这是首次从米曲霉中报道糖苷水解酶家族 43β-木糖苷酶。为了对该酶进行表征，将重组酶在大肠杆菌中进行了表达。与来自真菌的已知β-木糖苷酶不同，该酶没有表现出底物的模糊性，并且在碱性 pH 值下稳定。
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