Bakalova N, Petrova S, Kolev D
Department of Biochemistry, Faculty of Biology, University of Sofia, Bulgaria.
Pharmazie. 1996 Oct;51(10):761-4.
Three multiple forms (F-I, F-II and F-III) of xylanase produced from Aspergillus oryzae were established in the commercial preparation Luizym. Two methods were used (ion-exchange chromatography and polyacrylamide gel electrophoresis). The active xylanase fraction (F-III-II-I) was purified and characterized (pHopt 4.5; Topt 37 degrees C and molecular mass 38.5 kDa). The products of the enzymatic reaction were analysed by HPLC. The hydrolysis pattern showed that the xylanase was an exo-endo acting enzyme.
在商业制剂鲁氏酶（Luizym）中鉴定出了米曲霉产生的三种木聚糖酶多重形式（F-I、F-II和F-III）。采用了两种方法（离子交换色谱法和聚丙烯酰胺凝胶电泳法）。对活性木聚糖酶组分（F-III-II-I）进行了纯化和特性鉴定（最适pH 4.5；最适温度37℃；分子量38.5 kDa）。通过高效液相色谱法分析酶促反应产物。水解模式表明该木聚糖酶是一种外切-内切作用酶。
