Separation of enzymes from polyenzyme mixtures used in medicine and pharmacy. I. Isolation and characterization of xylanase from Aspergillus oryzae.

Three multiple forms (F-I, F-II and F-III) of xylanase produced from Aspergillus oryzae were established in the commercial preparation Luizym. Two methods were used (ion-exchange chromatography and polyacrylamide gel electrophoresis). The active xylanase fraction (F-III-II-I) was purified and characterized (pHopt 4.5; Topt 37 degrees C and molecular mass 38.5 kDa). The products of the enzymatic reaction were analysed by HPLC. The hydrolysis pattern showed that the xylanase was an exo-endo acting enzyme.