Department of Clinical Sciences, College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Theriogenology. 2010 May;73(8):1009-17. doi: 10.1016/j.theriogenology.2009.11.022. Epub 2010 Feb 2.
Bovine viral diarrhea virus (BVDV) has been shown to be associated with single transferable in vivo-derived bovine embryos despite washing and trypsin treatment. Hence, the primary objective was to evaluate the potential of BVDV to be transmitted via the intrauterine route at the time of embryo transfer. In vivo-derived bovine embryos (n=10) were nonsurgically collected from a single Bos tarus donor cow negative for BVDV. After collection and washing, embryos were placed into transfer media containing BVDV (SD-1; Type 1a). Each of the 10 embryos was individually loaded into an 0.25-mL straw, which was then nonsurgically transferred into the uterus of 1 of the 10 seronegative recipients on Day 0. The total quantity of virus transferred into the uterus of each of the 10 Bos tarus recipients was 878 cell culture infective doses to the 50% end point (CCID(50))/mL. Additionally, control heifers received 1.5 x 10(6) CCID(50) BVDV/.5 mL without an embryo (positive) or heat-inactivated BVDV (negative). The positive control heifer and all 10 recipients of virus-exposed embryos exhibited viremia by Day 6 and seroconverted by Day 15 after transfer. The negative control heifer did not exhibit a viremia or seroconvert. At 30 d after embryo transfer, 6 of 10 heifers in the treatment group were pregnant; however, 30 d later, only one was still pregnant. This fetus was nonviable and was positive for BVDV. In conclusion, the quantity of BVDV associated with bovine embryos after in vitro exposure can result in viremia and seroconversion of seronegative recipients after transfer into the uterus during diestrus.
牛病毒性腹泻病毒（BVDV）已被证明与单次可转移的体内衍生牛胚胎有关，尽管经过洗涤和胰蛋白酶处理。因此，主要目标是评估 BVDV 在胚胎转移时通过宫内途径传播的潜力。从一头 BVDV 阴性的单一公牛供体牛体内非手术采集了 10 个体内衍生的牛胚胎。采集和洗涤后，将胚胎放入含有 BVDV（SD-1；1a 型）的转移培养基中。将这 10 个胚胎中的每一个单独装入 0.25-mL 的吸管中，然后在第 0 天非手术转移到 10 个血清阴性受体中的 1 个子宫中。每个 Bos tarus 受体子宫内转移的病毒总量为 878 细胞培养感染剂量至 50%终点（CCID(50)/mL）。此外，对照小母牛接受 1.5 x 10(6)CCID(50)BVDV/0.5mL，无胚胎（阳性）或热失活 BVDV（阴性）。阳性对照小母牛和所有 10 个接受病毒暴露胚胎的受体在转移后第 6 天出现病毒血症，并在第 15 天血清转化。阴性对照小母牛未出现病毒血症或血清转化。在胚胎转移后 30 天，治疗组的 10 只小母牛中有 6 只怀孕；然而，30 天后，只有一只有效。这个胎儿没有生命力，并且对 BVDV 呈阳性。总之，在体外暴露后与牛胚胎相关的 BVDV 数量会导致在发情期转移到子宫后血清阴性受体的病毒血症和血清转化。
