Department of Animal Medicine, College of Animal Science and Technology, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, Anhui, PR China.
Anim Reprod Sci. 2010 Jun;119(3-4):275-81. doi: 10.1016/j.anireprosci.2010.01.003. Epub 2010 Jan 18.
Immunization using recombinant maltose binding protein-gonadotropin releasing hormone (MBP-GnRH6) altered both testicular development and transcription of the pituitary GnRH receptor (GnRHR) gene in boars. Scrotal measurement and blood samples were taken at 4-week interval after immunization at 9 weeks of age. The concentrations of testosterone and anti-GnRH antibodies in serum were determined by radioimmunoassay and enzyme-linked immunosorbent assay, respectively. The results showed that active immunization with MBP-GnRH6 increased the serum concentration of anti-GnRH antibodies (P<0.05) and reduced the serum concentration of testosterone (P<0.05) as compared with MBP controls. At 25 weeks of age, boars were sacrificed and testes were evaluated histologically. Testicular development was suppressed in the MBP-GnRH6 immunized animals as compared with MBP immunized boars. MBP-GnRH6 immunized pigs exhibited mounting behavior 4 weeks later than MBP immunized boars. No mature spermatozoa were observed from MBP-GnRH6 immunized animals. By real-time quantitative PCR analysis, the amount of GnRHR mRNA in the pituitary tissue was found to be significantly lower in MBP-GnRH6 immunized animals than in controls (P<0.05). These data demonstrate that recombinant MBP-GnRH6 was effective in immunological castration in boars.
免疫重组麦芽糖结合蛋白-促性腺激素释放激素（MBP-GnRH6）改变了公猪的睾丸发育和垂体 GnRH 受体（GnRHR）基因的转录。在 9 周龄时进行免疫接种后，每 4 周进行一次阴囊测量和采血。通过放射免疫测定和酶联免疫吸附测定分别测定血清中睾酮和抗 GnRH 抗体的浓度。结果表明，与 MBP 对照组相比，MBP-GnRH6 主动免疫增加了血清中抗 GnRH 抗体的浓度（P<0.05），降低了血清中睾酮的浓度（P<0.05）。在 25 周龄时，公猪被处死并进行组织学评估。与 MBP 免疫公猪相比，MBP-GnRH6 免疫动物的睾丸发育受到抑制。MBP-GnRH6 免疫猪的性行为表现出比 MBP 免疫猪晚 4 周。在 MBP-GnRH6 免疫动物中未观察到成熟的精子。通过实时定量 PCR 分析，发现 MBP-GnRH6 免疫动物的垂体组织中 GnRHR mRNA 的量明显低于对照组（P<0.05）。这些数据表明，重组 MBP-GnRH6 可有效免疫性去势公猪。
