Li Yunsheng, Liu Ya, Su Shiping, Pu Yong, Zhang Xiaorong, Fang Fugui
Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding,College of Animal Sciences and Technology,Anhui Agricultural University,130 Changjiang West Road,Hefei,Anhui 230036,China.
College of Animal Sciences and Technology,Anhui Agricultural University,130 Changjiang West Road,Hefei,Anhui 230036,China.
Zygote. 2015 Feb;23(1):125-35. doi: 10.1017/S0967199413000397. Epub 2013 Sep 2.
The aim of this study was to evaluate and to compare testicular tissue in immunized and control boars. Eighteen male piglets, aged 12 weeks, were vaccinated twice intramuscularly with a maltose-binding protein-gonadotropin-releasing hormone I hexamer peptide (MBP-GnRH-I6). Blood samples were taken at 12, 18, 21 and 24 weeks of age. Serum concentrations of testosterone and GnRH-I antibodies were determined by radioimmunoassay. The pigs were sacrificed 6 weeks after the second immunization. Testicular weight and size were recorded and tissue samples were collected for histological examination. The results demonstrated that active immunization against MBP-GnRH-I6 increased serum GnRH-I antibody levels (P < 0.05) and reduced serum concentrations of testosterone (P < 0.05) when compared with controls. Histological studies performed on testicular tissue revealed clear signs of atrophy in the MBP-GnRH-I6 immunized pigs, and a significant reduction (P < 0.05) in paired testes weight and size were seen in the treated boars. Microscopically, the mean diameter of the seminiferous tubules was markedly reduced (P < 0.01). Spermatogonia were visible, as well as few spermatocytes, but no spermatozoa were detected in the seminiferous tubules. Ultramicroscopic analysis of testicular tissue revealed an increase in the thickness of the basement membrane and extensive damage in the cell organelles of the treated animals, including small spermatogonial size, decreased number of mitochondria and endoplasmic reticulum in the primary spermatocyte and spermatid, a shallow hollow for nuclear membranes in Sertoli cells and mitochondrial vacuolation in Leydig cells. We conclude that MBP-GnRH-I6 induces severe atrophy in the testes of immunized boars.
本研究的目的是评估和比较免疫公猪与对照公猪的睾丸组织。18头12周龄的雄性仔猪通过肌肉注射麦芽糖结合蛋白 - 促性腺激素释放激素I六聚体肽（MBP - GnRH - I6）进行两次免疫接种。在12、18、21和24周龄时采集血样。通过放射免疫测定法测定血清睾酮浓度和GnRH - I抗体。在第二次免疫接种后6周处死猪。记录睾丸重量和大小，并收集组织样本进行组织学检查。结果表明，与对照组相比，针对MBP - GnRH - I6的主动免疫增加了血清GnRH - I抗体水平（P < 0.05）并降低了血清睾酮浓度（P < 0.05）。对睾丸组织进行的组织学研究显示，MBP - GnRH - I6免疫接种的猪出现明显的萎缩迹象，并且在处理过的公猪中，成对睾丸的重量和大小显著降低（P < 0.05）。显微镜下，生精小管的平均直径明显减小（P < 0.01）。可见精原细胞以及少量精母细胞，但在生精小管中未检测到精子。睾丸组织的超微结构分析显示，处理动物的基底膜厚度增加，细胞器广泛受损，包括精原细胞体积小、初级精母细胞和精子细胞中线粒体和内质网数量减少、支持细胞中核膜浅凹陷以及间质细胞中线粒体空泡化。我们得出结论，MBP - GnRH - I6可诱导免疫接种公猪的睾丸严重萎缩。
