International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58, Rinkuourai-Kita, Izumisano, Osaka, Japan.
Microbiol Res. 2010 Oct 20;165(8):639-48. doi: 10.1016/j.micres.2009.12.001. Epub 2010 Feb 2.
A bacterial disease was reported from gilthead sea bream (Sparus aurata) within a hatchery environment in Malta. Symptoms included complete erosion of tail, infection in the eye, mucous secretion and frequent mortality. A total of 540 strains were initially isolated in marine agar from different infected body parts and culture water sources. Subsequently 100 isolates were randomly selected, identified biochemically and all were found to be Vibrio harveyi-related organisms; finally from 100 isolates a total of 13 numbers were randomly selected and accurately identified as V. harveyi by 16S rRNA gene sequencing and species-specific PCR. Ribotyping of these strains with HindIII revealed total of six clusters. In vivo challenge study with representative isolates from each cluster proved two clusters each were highly pathogenic, moderately pathogenic and non-pathogenic. All 13 isolates were positive for hemolysin gene, a potential virulence factor. Further analysis revealed probably a single copy of this gene was encoded in all isolates, although not in the same locus in the genome. Although V. harveyi was reported to be an important pathogen for many aquatic organisms, to our knowledge this might be the first report of disease caused by V. harveyi and their systematic study in the sea bream hatchery from Malta.
在马耳他的一个孵化场环境中，报道了一种来自金头鲷（Sparus aurata）的细菌性疾病。症状包括尾巴完全侵蚀、眼睛感染、黏液分泌和频繁死亡。最初从不同感染的身体部位和养殖水源的海琼脂中分离出 540 株细菌。随后随机选择了 100 株进行生化鉴定，所有菌株均被鉴定为哈维氏弧菌相关生物；最后从 100 株菌株中随机选择了 13 株，并通过 16S rRNA 基因测序和种特异性 PCR 准确鉴定为哈维氏弧菌。用 HindIII 对这些菌株进行核糖体分型，共发现 6 个聚类。对每个聚类的代表性分离株进行体内攻毒研究表明，有 2 个聚类为高致病性、中等致病性和非致病性。所有 13 株分离株的溶血基因均为阳性，这是一种潜在的毒力因子。进一步分析表明，尽管在基因组中的相同位置不存在，但所有分离株都可能编码该基因的单个拷贝。尽管哈维氏弧菌被报道为许多水生生物的重要病原体，但据我们所知，这可能是马耳他金头鲷孵化场由哈维氏弧菌引起的疾病及其系统研究的首次报道。
