School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
Biosens Bioelectron. 2010 Apr 15;25(8):1989-93. doi: 10.1016/j.bios.2010.01.009. Epub 2010 Jan 18.
A facile and high performance biosensing platform using aligned carbon nanotubes on quartz substrate is reported in this communication. Single walled carbon nanotubes are grown on quartz substrates by a chemical vapor deposition process and are characterized with field emission scanning electron microscopy and atomic force microscopy in order to verify the quality of the material. The quartz substrate is then directly used as a biosensor in a field effect transistor configuration. In order to demonstrate the sensing capabilities of the fabricated sensor devices, electronic detection of prostate specific antigen, a potential cancer biomarker, is carried out by adopting liquid gated configuration. A conductivity change due to the specific binding of target antigen with the immobilized receptor antibody demonstrates the sensing capabilities of the fabricated device. Sub-nM detection sensitivities have been obtained using the adopted direct immunoassay approach, which shows that the device responds to clinically relevant concentration regimes.
本文报道了一种基于石英衬底上定向碳纳米管的简便、高性能生物传感平台。通过化学气相沉积工艺在石英衬底上生长单壁碳纳米管，并通过场发射扫描电子显微镜和原子力显微镜对其进行了表征，以验证材料的质量。然后，石英衬底直接用作场效应晶体管结构中的生物传感器。为了演示所制造的传感器设备的传感能力，通过采用液体门控配置，对前列腺特异性抗原（一种潜在的癌症生物标志物）进行了电子检测。由于目标抗原与固定化受体抗体的特异性结合导致的电导率变化证明了所制造器件的传感能力。采用直接免疫测定法获得了亚纳摩尔检测灵敏度，表明该器件可响应临床相关的浓度范围。
