利用实时 PCR 和 MAP1506 基因座高分辨率熔解分析快速鉴定和区分分枝杆菌副结核亚种。
Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus.
机构信息
Centro de Vigilancia Sanitaria Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.
出版信息
J Clin Microbiol. 2010 Apr;48(4):1474-7. doi: 10.1128/JCM.02484-09. Epub 2010 Feb 3.
Abstract
High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
摘要
高分辨率熔解（HRM）分析可以通过比较实时 PCR 扩增产生的扩增子的熔解曲线来识别序列多态性。我们描述了该技术在鉴定禽分枝杆菌亚种副结核分枝杆菌 I、II 和 III 型中的应用。HRM 方法基于 MAP1506 中的种特异性核苷酸序列，MAP1506 是 PPE（脯氨酸-脯氨酸-谷氨酸）基因家族的一个成员。
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