Department of Cell Biology, Poznan University of Medical Sciences, Poznan, Poland.
Int J Gynecol Cancer. 2010 Jan;20(1):92-101. doi: 10.1111/IGC.0b013e3181bbe933.
Recent studies propose the role of gonadotropins in the development and growth of endometrial carcinoma. The present research was undertaken to establish the expression of human chorionic gonadotropin (hCG), gonadotropin-releasing hormones 1 (GnRH1 and GnRH2, respectively) and their receptors in endometrial hyperplasias and carcinoma.
The expression of analyzed genes in endometrial carcinoma and hyperplasia with and without atypia was evaluated using reverse transcriptase polymerase chain reaction and immunohistochemistry.
The results of the experiments demonstrated the presence of hCG and GnRH1 at both messenger RNA and protein levels in endometrial carcinoma and atypical hyperplasia. Noncancerous tissue and hyperplasia without atypia demonstrated the lack of these gene coexpressions. The expression of GnRH2, LH/hCGR, and GnRHRs was heterogeneous, and the study molecules were found only in part of the analyzed tissues. The presence of hCG and GnRH1 and their receptors in cancer tissue and atypical hyperplasia suggests autocrine/paracrine action of hormones regulating the endometrial carcinoma cell proliferation.
The interaction between the hCG and LH/hCGR in endometrial tissue might stimulate cell growth and promote neoangiogenesis, whereas GnRHs, by binding to their receptors, could be responsible for the antiproliferative effect and stimulation of apoptosis. The identification of differences in the expression profile of the analyzed genes could be relevant for better understanding of the development of endometrial carcinomas and could be useful in clinical diagnostics.
最近的研究表明促性腺激素在子宫内膜癌的发生和发展中起作用。本研究旨在建立人绒毛膜促性腺激素（hCG）、促性腺激素释放激素 1（GnRH1 和 GnRH2）及其受体在子宫内膜增生和癌中的表达。
采用逆转录聚合酶链反应和免疫组织化学方法检测分析基因在子宫内膜癌和伴或不伴非典型性增生中的表达。
实验结果表明 hCG 和 GnRH1 在子宫内膜癌和非典型性增生的信使 RNA 和蛋白水平上均有表达。非癌组织和无异型性增生缺乏这些基因的共表达。GnRH2、LH/hCGR 和 GnRHRs 的表达具有异质性，研究分子仅存在于部分分析组织中。hCG 和 GnRH1 及其受体在癌组织和非典型性增生中的存在提示激素的自分泌/旁分泌作用调节子宫内膜癌细胞的增殖。
hCG 和 LH/hCGR 在子宫内膜组织中的相互作用可能刺激细胞生长并促进新血管生成，而 GnRHs 通过与其受体结合可能对增殖产生抑制作用并刺激细胞凋亡。分析基因表达谱的差异可能有助于更好地了解子宫内膜癌的发生，并可能有助于临床诊断。
