Department of Gastroenterology, First Hospital, Jilin University, 130021, Changchun, China.
Dig Dis Sci. 2010 Oct;55(10):2805-13. doi: 10.1007/s10620-009-1117-5. Epub 2010 Feb 4.
Experiments have reported that granulocyte colony stimulating factor (G-CSF) can mobilize stem cells. However, few studies have examined the effect of G-CSF on bone marrow mononuclear cell (BMMC) mobilization, in particular regarding their capability to home to acutely injured liver.
The aim of this study was to evaluate the effort of G-CSF on BMMC homing to the liver following chemically-induced hepatic failure.
BMMC were isolated from mice, pre-labeled with PKH26 and infused into the mice in which hepatic injury had been induced followed by administration of G-CSF or vehicle. Livers were studied by fluorescent microscopy after transplantation of pre-labeled BMMC.
PKH26 labeled cells were found in liver tissue at 102 ± 10 cells/high power field in the BMMC+G-CSF group and 30 ± 5 cells/high power field in the BMMC group, but none in the G-CSF group and the control group (P < 0.05). In the former two groups the majority of PKH26 labeled cells colocalized with proliferative cell nuclear antigen (PCNA). The number of PCNA positive cells in the BMMC+G-CSF group was 20 ± 4 cells/high power field, while in the BMMC group it was 14 ± 2 cells/high power field, in the G-CSF group 12 ± 2 cells/high power field, and 8 ± 1 cells/high power field in the control group. Moreover, albumin expression was increased in the BMMC+G-CSF treated group (149 ± 7/high power field) relative to the BMMC group (48 ± 6/high power field), the G-CSF group (44 ± 5/high power field) and the vehicle group (30 ± 6/high power field), with the former three groups showing elevated levels as compared to vehicle control (30 ± 6) (P < 0.05).
Transplanted BMMC may home to injured liver, which appears to be enhanced by G-CSF administration.
实验已经报道粒细胞集落刺激因子（G-CSF）可以动员干细胞。然而，很少有研究探讨 G-CSF 对骨髓单个核细胞（BMMC）动员的影响，特别是关于它们归巢到急性受损肝脏的能力。
本研究旨在评估 G-CSF 对化学诱导肝衰竭后 BMMC 归巢到肝脏的作用。
从小鼠中分离 BMMC，用 PKH26 预先标记，然后输注到已经诱导肝损伤的小鼠中，同时给予 G-CSF 或载体。移植预标记的 BMMC 后，通过荧光显微镜研究肝脏。
在 BMMC+G-CSF 组中，PKH26 标记的细胞在肝组织中发现为 102 ± 10 个/高倍视野，在 BMMC 组中发现为 30 ± 5 个/高倍视野，但在 G-CSF 组和对照组中均未发现（P < 0.05）。在前两组中，大多数 PKH26 标记的细胞与增殖细胞核抗原（PCNA）共定位。BMMC+G-CSF 组中 PCNA 阳性细胞的数量为 20 ± 4 个/高倍视野，BMMC 组为 14 ± 2 个/高倍视野，G-CSF 组为 12 ± 2 个/高倍视野，对照组为 8 ± 1 个/高倍视野。此外，在 BMMC+G-CSF 处理组中白蛋白表达增加（149 ± 7/高倍视野），与 BMMC 组（48 ± 6/高倍视野）、G-CSF 组（44 ± 5/高倍视野）和载体组（30 ± 6/高倍视野）相比，前三个组的水平均高于载体对照组（30 ± 6）（P < 0.05）。
移植的 BMMC 可能归巢到受损的肝脏，而 G-CSF 的给予似乎增强了这一作用。
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