Division of Plastic and Reconstructive Surgery, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Laryngoscope. 2010 Mar;120(3):546-51. doi: 10.1002/lary.20797.
OBJECTIVES/HYPOTHESIS: Mesenchymal stem cells (MSCs) originally isolated from bone marrow (BM), are fibroblast-looking cells that are now assumed to be present in the stromal component of many tissues. MSCs are characterized by a certain set of criteria, including their growth culture characteristics, a combination of cell surface markers, and the ability to differentiate along multiple mesenchymal tissue lineages. We hypothesized that human vocal fold fibroblasts (hVFF) isolated from the lamina propria meet the criteria established to define MSCs and are functionally similar to MSCs derived from BM and adipose tissue.
In vitro study.
hVFF were previously derived from human vocal fold tissues. MSCs were derived from adipose tissue (AT), and BM of healthy donors based on their attachment to culture dishes and their morphology and expanded in culture. Cells were analyzed for standard cell surface markers identified on BM-derived MSCs and the ability to differentiate into cells of mesenchymal lineage (i.e., fat, bone, and cartilage). We investigated the immunophenotype of these cells before and after interferon-gamma (INF-gamma) stimulation.
hVFF displayed cell surface markers and multipotent differentiation capacity characteristic of MSCs. Furthermore, these cells exhibited similar patterns of expression of human leukocyte antigen and costimulatory molecules, after stimulation with INF-gamma compared to MSCs derived from BM and AT.
Based on our findings, hVFF derived from lamina propria have the same cell surface markers, immunophenotypic characteristics, and differentiation potential as BM- and AT-derived MSCs. We propose that vocal fold fibroblasts are MSCs resident in the vocal fold lamina propria.
目的/假设：最初从骨髓（BM）中分离出来的间充质干细胞（MSCs）是看起来像成纤维细胞的细胞，现在被认为存在于许多组织的基质成分中。MSCs 的特征是具有一定的标准，包括其生长培养特性、细胞表面标志物的组合以及沿多个间充质组织谱系分化的能力。我们假设从固有层分离的人声带成纤维细胞（hVFF）符合定义 MSCs 的标准，并且在功能上类似于源自 BM 和脂肪组织的 MSCs。
体外研究。
hVFF 先前从人声带组织中分离得到。MSCs 源自健康供体的脂肪组织（AT）和 BM，基于其对培养皿的附着及其形态，并在培养中进行扩增。分析细胞的标准 BM 衍生 MSC 上的细胞表面标志物，并分析其分化为间充质谱系细胞（即脂肪、骨骼和软骨）的能力。我们在干扰素-γ（INF-γ）刺激前后研究了这些细胞的免疫表型。
hVFF 表现出 MSC 特有的细胞表面标志物和多能分化能力。此外，与源自 BM 和 AT 的 MSC 相比，这些细胞在受到 INF-γ刺激后，表现出类似的人类白细胞抗原和共刺激分子表达模式。
根据我们的发现，源自固有层的 hVFF 具有与源自 BM 和 AT 的 MSC 相同的细胞表面标志物、免疫表型特征和分化潜能。我们提出，声带成纤维细胞是驻留在声带固有层中的 MSC。
