Department of Otorhinolaryngology-Head and Neck Surgery, Louisiana State University Health Sciences Center, New Orleans, Louisiana 70112, USA.
Laryngoscope. 2010 Mar;120(3):443-9. doi: 10.1002/lary.20679.
OBJECTIVES/HYPOTHESIS: AlloDerm (LifeCell Corp., Branchburg, NJ) is commonly employed for reconstruction of ablative soft tissue and mucosal defects following surgical resections. Although devoid of growth factors, AlloDerm may serve as an adhesive matrix for binding of growth factors, increasing local angiogenesis, and wound healing. We hypothesized that AlloDerm would enhance angiogenesis and might be altered with autologous blood products to enhance initiation of the angiogenic response.
We used a human placental vein in a fibrin-thrombin clot-based angiogenesis model. Four groups, human placental vein (HPVM), HPVM with AlloDerm, HPVM with AlloDerm plus platelet-poor plasma, and HPVM with AlloDerm plus platelet-rich plasma were evaluated. Endothelial cell growth was evaluated visually (40x). Hematoxylin and eosin staining and immunofluorescent staining for growth within the AlloDerm matrix were also performed. To assess human umbilical vein endothelial cell (HUVEC) sites of attachment to AlloDerm, we incubated HUVEC cells with AlloDerm for a period of 2 weeks and evaluated attachment with anti-factor VIII immunofluorescence.
Angiogenic initiation decreased in the combined placental vein with AlloDerm group (P < .0001 at day 7, 14, 21). Additionally, initiation in the AlloDerm plus platelet-poor plasma group was significantly better than the AlloDerm alone group when placentas 2 and 3 were compared (P < .0001). On hematoxylin and eosin staining and immunofluorescent factor VIII staining, no endothelial growth into the AlloDerm was noted in the samples analyzed.
AlloDerm may be enriched with platelet-poor plasma to stimulate greater initiation and wound healing; however, AlloDerm inhibits angiogenic initiation in this model.
目的/假设：AlloDerm（LifeCell Corp.，新泽西州布兰奇伯勒）常用于重建手术切除后消融的软组织和粘膜缺损。尽管没有生长因子，但 AlloDerm 可以作为结合生长因子的粘合基质，增加局部血管生成和伤口愈合。我们假设 AlloDerm 会增强血管生成，并且可以通过与自体血液制品结合来改变，以增强血管生成反应的启动。
我们使用纤维蛋白-凝血酶凝块为基础的血管生成模型中的人胎盘静脉。将 4 组人胎盘静脉（HPVM）、HPVM 加 AlloDerm、HPVM 加血小板贫血浆和 HPVM 加富含血小板的血浆进行评估。用 40x 观察内皮细胞生长。还进行了苏木精和伊红染色以及 AlloDerm 基质内生长的免疫荧光染色。为了评估人脐静脉内皮细胞（HUVEC）附着在 AlloDerm 上的部位，我们将 HUVEC 细胞与 AlloDerm 孵育 2 周，并通过抗因子 VIII 免疫荧光评估附着。
联合胎盘静脉与 AlloDerm 组的血管生成启动减少（第 7、14、21 天 P <.0001）。此外，当比较胎盘 2 和 3 时，AlloDerm 加血小板贫血浆组的启动明显优于单独使用 AlloDerm 组（P <.0001）。在苏木精和伊红染色和免疫荧光因子 VIII 染色中，在分析的样本中未发现内皮细胞进入 AlloDerm。
AlloDerm 可以用血小板贫血浆富集以刺激更大的启动和伤口愈合；然而，在该模型中，AlloDerm 抑制血管生成启动。
