Department of Neurosurgery, University Medical Centre Ljubljana, Slovenia Laboratory Centre of Medical Faculty Maribor, Maribor, Slovenia.
Int Wound J. 2013 Jun;10(3):321-8. doi: 10.1111/j.1742-481X.2012.00980.x. Epub 2012 Apr 9.
New therapeutic approaches for wound treatment are evolving. Non healing wounds in oncology and after trauma may be cured by a novel technique of tissue augmentation with soft tissue fillers. The principle resides in filling the wound with collagen filler in order to seal the defect and promote healing. Successful angiogenesis forms the basis of tissue filler survival and determines the outcome of the healing process. During this study, basic data about endothelial cell invasion into collagen-made substratum was collected that could be used for neoangiogenesis studies in tissue augmentation techniques for large wound defect treatment. In the in vitro assay, the human umbilical vein endothelial cells (HUVEC) grow into a three-dimensional framework of collagenous tissue fillers, forming the basic step for angiogenesis. After heparins were used as chemotactic agents, a typical bell-shaped relationship between chemotaxis and agent concentrations was found. Significant cell infiltration was present in the assays with chemotactic agents. These observations support the potential for tissue augmentation with soft tissue fillers that could be used in acute and chronic non healing traumatic and oncology wounds after extensive surgical resections and radiotherapy.
新的创伤治疗方法正在不断发展。肿瘤和创伤后无法愈合的伤口可以通过一种新的软组织填充组织增强技术来治愈。其原理是用胶原蛋白填充剂填充伤口，以封闭缺损并促进愈合。成功的血管生成是组织填充物存活的基础，并决定了愈合过程的结果。在这项研究中，收集了关于内皮细胞侵入胶原蛋白基质的基本数据，这些数据可用于研究组织填充技术在治疗大的创伤缺损时的新血管生成。在体外试验中，人脐静脉内皮细胞（HUVEC）生长到胶原组织填充剂的三维框架中，形成血管生成的基本步骤。使用肝素作为趋化剂后，发现趋化作用与药剂浓度之间存在典型的钟形关系。趋化剂试验中有明显的细胞浸润。这些观察结果支持使用软组织填充剂进行组织增强的潜力，这种方法可用于广泛手术切除和放疗后的急性和慢性创伤和肿瘤无法愈合的伤口。
