Textile Department, Center of Excellence in Textile, Amirkabir University of Technology, Tehran, Iran.
J Liposome Res. 2010 Dec;20(4):323-9. doi: 10.3109/08982100903544177. Epub 2010 Feb 4.
Silver nanoparticles were synthesized and stabilized by a simple, environment-friendly method in a liposomes structure. Liposomes were prepared by facing lecithin to the aqueous-phase solutions while stirring vigorously. The ratio of lecithin concentration to silver nitrate (K(Lec/Ag) = [Lecithin]/[AgNO(3)]) is the influencing factor in the synthesis of silver nanoparticles. The stability, size distribution, and antibacterial properties of synthesized silver nanoparticles were studied by ultraviolet (UV)-visible, dynamic light scattering, and antibacterial assay. The UV spectra indicated a single symmetric extinction peak at 400 nm, confirming the spherical shape of the synthesized silver nanoparticles. A high K(Lec/Ag) value leads to a reduction in the intensity of extinction spectra and increases the size of Ag-liposomes nanocomposites. The large Ag-liposomes nanocomposites are transformed to the smaller Ag-liposomes nanocomposites (from 342 to 190 nm) due to sonication treatment. The stabilized silver nanoparticles with various lecithin concentrations showed a good antibacterial activity against Staphylococcus aureus, a Gram-positive bacterium, and Escherichia coli, a Gram-negative bacterium.
采用简单、环保的方法在脂质体结构中合成并稳定了银纳米粒子。脂质体通过在剧烈搅拌下使卵磷脂面向水相溶液来制备。卵磷脂浓度与硝酸银的比值（K(Lec/Ag) = [卵磷脂]/[AgNO(3)])是影响银纳米粒子合成的因素。通过紫外（UV）-可见分光光度法、动态光散射和抗菌试验研究了合成银纳米粒子的稳定性、粒径分布和抗菌性能。紫外光谱在 400nm 处显示出单一的对称消光峰，证实了所合成的银纳米粒子的球形形状。高 K(Lec/Ag) 值会降低消光光谱的强度并增加 Ag-脂质体纳米复合材料的尺寸。由于超声处理，大的 Ag-脂质体纳米复合材料转化为较小的 Ag-脂质体纳米复合材料（从 342nm 到 190nm）。具有不同卵磷脂浓度的稳定化银纳米粒子对革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌大肠杆菌均显示出良好的抗菌活性。
