Dipartimento di Chimica, Sezione di Chimica Generale, Università di Pavia, viale Taramelli, 12-27100 Pavia, Italy.
Langmuir. 2011 Aug 2;27(15):9165-73. doi: 10.1021/la201200r. Epub 2011 Jul 7.
In the present work, we describe a simple procedure to produce biomimetically coated silver nanoparticles (Ag NPs), based on the postfunctionalization and purification of colloidal silver stabilized by citrate. Two biological capping agents have been used (cysteine Cys and glutathione GSH). The composition of the capped colloids has been ascertained by different techniques and antibacterial tests on GSH-capped Ag NPs have been conducted under physiological conditions, obtaining values of Minimum Inhibitory Concentration (MIC) of 180 and 15 μg/mL for Staphylococcus aureus and Escherichia coli, respectively. The antibacterial activity of these GSH capped NPs can be ascribed to the direct action of metallic silver NPs, rather than to the bulk release of Ag(+).
在本工作中，我们描述了一种基于胶体银的后功能化和纯化来制备仿生包覆银纳米粒子（Ag NPs）的简单方法，该胶体由柠檬酸稳定。我们使用了两种生物封端剂（半胱氨酸 Cys 和谷胱甘肽 GSH）。通过不同的技术确定了被包覆胶体的组成，并在生理条件下对 GSH 包覆的 Ag NPs 进行了抗菌测试，分别得到了对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度（MIC）值为 180 和 15 μg/mL。这些 GSH 封端 NPs 的抗菌活性可以归因于金属银 NPs 的直接作用，而不是 Ag(+) 的大量释放。
