来自腺病毒 5 载体表达的杜克雷嗜血杆菌细胞致死扩张毒素的 CdtB 组分的毒性活性。

Toxic activity of the CdtB component of Haemophilus ducreyi cytolethal distending toxin expressed from an adenovirus 5 vector.

机构信息

Department of Microbiology and Immunology, Institute of Biomedicine, The Sahlgrenska Academy, Göteborg University, Göteborg, Sweden.

出版信息

APMIS. 2010 Feb;118(2):143-9. doi: 10.1111/j.1600-0463.2009.02573.x.

DOI:10.1111/j.1600-0463.2009.02573.x

PMID:20132178

Abstract

The Haemophilus ducreyi cytolethal distending toxin (HdCDT) catalytic subunit CdtB has DNase-like activity and mediates DNA damage after its delivery into target cells. We constructed a replication-deficient adenovirus type 5 (Ad5) vector expressing CdtB and investigated the toxic properties of this vector on HeLa cells. Ad5CdtB caused loss of cell viability, morphologic changes, and cell cycle arrest, findings similar to HdCDT intoxication. This confirmed that CdtB is responsible for the toxicity of the holotoxin when expressed in cells following transduction by an adenoviral vector, and indicated a possible potential of this novel strategy in studies of activity of intracellular products and in gene therapy of cancer.

摘要

杜克雷嗜血杆菌细胞致死扩张毒素（HdCDT）的催化亚基 CdtB 具有 DNA 酶样活性，并在其递送至靶细胞后介导 DNA 损伤。我们构建了表达 CdtB 的复制缺陷型 5 型腺病毒（Ad5）载体，并研究了该载体对 HeLa 细胞的毒性特性。Ad5CdtB 导致细胞活力丧失、形态改变和细胞周期停滞，与 HdCDT 中毒的发现相似。这证实了在腺病毒载体转导后细胞中表达时，CdtB 负责全毒素的毒性，并且表明该新型策略在细胞内产物活性研究和癌症基因治疗中具有潜在的可能性。