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基于紫外诱导荧光光谱的蓝绿区提高单子叶和双子叶植物杂草防治的鉴别能力。

Improved discrimination between monocotyledonous and dicotyledonous plants for weed control based on the blue-green region of ultraviolet-induced fluorescence spectra.

机构信息

Horticultural R&D Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, QC, Canada, J3B 3E6.

出版信息

Appl Spectrosc. 2010 Jan;64(1):30-6. doi: 10.1366/000370210790572106.

Abstract

Precision weeding by spot spraying in real time requires sensors to discriminate between weeds and crop without contact. Among the optical based solutions, the ultraviolet (UV) induced fluorescence of the plants appears as a promising alternative. In a first paper, the feasibility of discriminating between corn hybrids, monocotyledonous, and dicotyledonous weeds was demonstrated on the basis of the complete spectra. Some considerations about the different sources of fluorescence oriented the focus to the blue-green fluorescence (BGF) part, ignoring the chlorophyll fluorescence that is inherently more variable in time. This paper investigates the potential of performing weed/crop discrimination on the basis of several large spectral bands in the BGF area. A partial least squares discriminant analysis (PLS-DA) was performed on a set of 1908 spectra of corn and weed plants over 3 years and various growing conditions. The discrimination between monocotyledonous and dicotyledonous plants based on the blue-green fluorescence yielded robust models (classification error between 1.3 and 4.6% for between-year validation). On the basis of the analysis of the PLS-DA model, two large bands were chosen in the blue-green fluorescence zone (400-425 nm and 425-490 nm). A linear discriminant analysis based on the signal from these two bands also provided very robust inter-year results (classification error from 1.5% to 5.2%). The same selection process was applied to discriminate between monocotyledonous weeds and maize but yielded no robust models (up to 50% inter-year error). Further work will be required to solve this problem and provide a complete UV fluorescence based sensor for weed-maize discrimination.

摘要

实时精确喷药除草需要传感器在不接触的情况下区分杂草和作物。在基于光学的解决方案中,植物的紫外(UV)诱导荧光似乎是一种很有前途的替代方法。在第一篇论文中,根据完整的光谱,证明了区分玉米杂种、单子叶植物和双子叶植物杂草的可行性。关于荧光源的一些考虑将研究重点放在蓝绿光(BGF)部分,而忽略了叶绿素荧光,因为叶绿素荧光在时间上的变化更大。本文研究了基于 BGF 区域的几个大光谱带进行杂草/作物区分的潜力。对 1908 个玉米和杂草植物的光谱进行了三年和各种生长条件下的偏最小二乘判别分析(PLS-DA)。基于蓝绿光的单子叶植物和双子叶植物的区分产生了稳健的模型(年际验证的分类错误在 1.3%到 4.6%之间)。基于 PLS-DA 模型的分析,选择了蓝绿光区域中的两个大带宽(400-425nm 和 425-490nm)。基于这两个波段信号的线性判别分析也提供了非常稳健的年际结果(分类错误率为 1.5%至 5.2%)。同样的选择过程也适用于区分单子叶杂草和玉米,但没有产生稳健的模型(年际误差高达 50%)。需要进一步的工作来解决这个问题,并提供一个完整的基于 UV 荧光的传感器来区分杂草和玉米。

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