Department of Food and Nutrition, College of Medical Life Science, Silla University, Busan 617-736, Korea.
BMB Rep. 2010 Jan;43(1):9-16. doi: 10.5483/bmbrep.2010.43.1.009.
The promoters of OsCaM1 and OsCaM3 were characterized after sequencing and fused to the reporter gene, GUS. The constructs were then transformed into the tobacco plant. Histochemical analysis of GUS showed different expression patterns in pOsCaM1::GUS and pOsCaM3:: GUS transgenic plants. The expression of pOsCaM1::GUS in 4- to 15-day-old seedlings in particular was observed only in the root, while the expression of pOsCaM3::GUS was detected in both the cotyledons and root. Also, pRCaM1::GUS was detected in all the tissues surrounding the root system, while the presence of pOsCaM3::GUS was observed in the root, except in the root meristem. However, in mature transgenic plants, the expression of pOsCaM1::GUS and OsRCaM3::GUS was scarcely detected. Under wounding stress, the GUS activity of pOsCaM1 and pOsCaM3 was strongly induced, and the activity of pOsCaM3 especially, was retained for long periods. In the phloem, pOsCaM3 activity induced by hormone treatments and abiotic stresses was also identified. [BMB reports 2010; 43(1): 9-16].
启动子 OsCaM1 和 OsCaM3 测序后与报告基因 GUS 融合。然后将构建体转化到烟草植物中。GUS 的组织化学分析显示，在 pOsCaM1::GUS 和 pOsCaM3::GUS 转基因植物中，GUS 的表达模式不同。特别是在 4 至 15 天大的幼苗中，观察到 pOsCaM1::GUS 的表达仅在根中，而 pOsCaM3::GUS 的表达在子叶和根中均被检测到。此外，pRCaM1::GUS 在根系周围的所有组织中均被检测到，而 pOsCaM3::GUS 除在根尖端外，在根中也被检测到。然而，在成熟的转基因植物中，pOsCaM1::GUS 和 OsRCaM3::GUS 的表达几乎检测不到。在创伤应激下，pOsCaM1 和 pOsCaM3 的 GUS 活性被强烈诱导，特别是 pOsCaM3 的活性持续很长时间。在韧皮部中，还鉴定了激素处理和非生物胁迫诱导的 pOsCaM3 活性。[BMB 报告 2010；43(1): 9-16]。
