Medical & Bio-Material Research Center and Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Korea.
BMB Rep. 2010 Jan;43(1):29-33. doi: 10.5483/bmbrep.2010.43.1.029.
It is well known that PR/SET family members participate in transcriptional regulation via chromatin remodeling. PRDM10 might play an essential role in gene expression, but no such evidence has been observed so far. To assess PRDM10 expression at various stages of mouse development, we performed immunohistochemistry using available PRDM10 antibody. Embryos were obtained from three distinct developmental stages. At E8.5, PRDM10 expression was concentrated in the mesodermal and neural crest populations. As embryogenesis proceeded further to E13.5, PRMD10 expression was mainly in mesoderm-derived tissues such as somites and neural crest-derived populations such as the facial skeleton. This expression pattern was consistently maintained to the fetal growth period E16.5 and adult mouse, suggesting that PRDM10 may function in tissue differentiation. Our study revealed that PRDM10 might be a transcriptional regulator for normal tissue differentiation during mouse embryonic development. [BMB reports 2010; 43(1): 29-33].
众所周知，PR/SET 家族成员通过染色质重塑参与转录调控。PRDM10 可能在基因表达中发挥重要作用，但迄今为止尚未观察到这种证据。为了评估 PRDM10 在小鼠发育的不同阶段的表达情况，我们使用现有的 PRDM10 抗体进行了免疫组织化学分析。胚胎取自三个不同的发育阶段。在 E8.5，PRDM10 的表达集中在中胚层和神经嵴群体中。随着胚胎发育进一步进行到 E13.5，PRMD10 的表达主要在中胚层衍生的组织如体节和神经嵴衍生的组织如面部骨骼中。这种表达模式一直持续到胎儿生长阶段 E16.5 和成年小鼠，表明 PRDM10 可能在组织分化中发挥作用。我们的研究表明，PRDM10 可能是小鼠胚胎发育过程中正常组织分化的转录调节因子。[BMB 报告 2010；43(1): 29-33]。
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