Clinical Laboratory, Vetsuisse-Faculty, University of Zurich, Winterthurerstreet 260, CH-8057 Zurich, Switzerland.
Vet J. 2011 Mar;187(3):381-7. doi: 10.1016/j.tvjl.2010.01.005. Epub 2010 Feb 4.
A novel haematology analyser was evaluated for its use with feline samples. Complete blood cell counts, a five-part differential count, and reticulocyte numbers were determined, and the results compared with reference data. Coefficients of correlation, Passing-Bablok regression analysis and Bland-Altmann difference plots with biases and 95% limits of agreement are reported. Precision and linearity were also studied. The instrument demonstrated very low imprecision, and the tested range of linearity exceeded the reference ranges provided by the manufacturer. For all parameters except monocytes (r = 0.65), the analyser results correlated well with reference methods. Compared with the manual count of aggregated reticulocytes, the instrument showed good agreement with a positive bias. The optical platelet count correlated well with the manual chamber count. In conclusion the analyser was found to be highly reliable for the analysis of feline blood samples in a large veterinary laboratory.
一种新型血液学分析仪被评估用于猫科动物样本。进行了全血细胞计数、五分群白细胞分类计数和网织红细胞计数,并将结果与参考数据进行比较。报告了相关系数、Passing-Bablok 回归分析以及 Bland-Altmann 差异图及其偏差和 95%一致性界限。还研究了精密度和线性度。该仪器显示出非常低的不精密度,并且测试的线性范围超过了制造商提供的参考范围。除单核细胞外(r = 0.65),所有参数的分析仪结果与参考方法相关性良好。与手动聚集网织红细胞计数相比,该仪器显示出良好的一致性,具有正偏差。光学血小板计数与手动室计数相关性良好。总之,该分析仪在大型兽医实验室中分析猫科动物血液样本时被发现具有高度可靠性。