Laurent-Winter C, Soussi-Yanicostas N, Butler-Browne G S
Laboratoire d'électrophorèse bidimensionnelle, Institut Pasteur, Paris, France.
FEBS Lett. 1991 Mar 25;280(2):292-6. doi: 10.1016/0014-5793(91)80315-t.
Using a two-dimensional electrophoresis technique coupled with sensitive silver staining, we have investigated the chronology of appearance of the myosin light chain and tropomyosin isoforms during early stages of human quadriceps development. Our results show that slow myosin light chains and the slow tropomyosin isoform are not detected at 6 weeks of gestation. These isoforms transiently appear between 12.5 weeks and 15 weeks of gestation and then disappear. The slow myosin light chains are re-expressed at 31 weeks of gestation and the slow tropomyosin isoform later at 36 weeks of gestation, and normally remained expressed into the adulthood. Our study thus reveals a biphasic expression of the slow myosin light chains and the slow tropomyosin isoform in developing human quadriceps muscle.
利用二维电泳技术结合灵敏的银染法，我们研究了人股四头肌发育早期肌球蛋白轻链和原肌球蛋白亚型出现的时间顺序。我们的结果显示，在妊娠6周时未检测到慢肌球蛋白轻链和慢原肌球蛋白亚型。这些亚型在妊娠12.5周和15周之间短暂出现，然后消失。慢肌球蛋白轻链在妊娠31周时重新表达，慢原肌球蛋白亚型在妊娠36周时稍后重新表达，并正常持续表达至成年期。因此，我们的研究揭示了发育中的人股四头肌中慢肌球蛋白轻链和慢原肌球蛋白亚型的双相表达。
