细菌翻译延伸因子 EF-Tu 与肌动蛋白样 MreB 蛋白相互作用并共定位。
Bacterial translation elongation factor EF-Tu interacts and colocalizes with actin-like MreB protein.
机构信息
Mikrobiologie, Fakultät für Biologie, Universität Freiburg, 79104 Freiburg, Germany.
出版信息
Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3163-8. doi: 10.1073/pnas.0911979107. Epub 2010 Jan 28.
Abstract
We show that translation initiation factor EF-Tu plays a second important role in cell shape maintenance in the bacterium Bacillus subtilis. EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB, an actin-like cytoskeletal element setting up rod cell shape. The localization of MreB and of EF-Tu is interdependent, but in contrast to the dynamic MreB filaments, EF-Tu structures are more static and may serve as tracks for MreB filaments. In agreement with this idea, EF-Tu and MreB interact in vivo and in vitro. Lowering of the EF-Tu levels had a minor effect on translation but a strong effect on cell shape and on the localization of MreB, and blocking of the function of EF-Tu in translation did not interfere with the localization of MreB, showing that, directly or indirectly, EF-Tu affects the cytoskeletal MreB structure and thus serves two important functions in a bacterium.
摘要
我们发现翻译起始因子 EF-Tu 在枯草芽孢杆菌的细胞形态维持中发挥着第二个重要作用。EF-Tu 在细胞膜下呈螺旋状定位，并与 MreB 共定位，MreB 是一种肌动蛋白样细胞骨架成分，负责形成杆状细胞形态。MreB 和 EF-Tu 的定位是相互依赖的，但与动态的 MreB 纤维不同，EF-Tu 结构更具静态性，可能作为 MreB 纤维的轨道。这一观点与 EF-Tu 和 MreB 在体内和体外相互作用的实验结果一致。降低 EF-Tu 水平对翻译的影响较小，但对细胞形态和 MreB 的定位影响较大，并且阻断 EF-Tu 在翻译中的功能并不干扰 MreB 的定位，这表明 EF-Tu 直接或间接地影响细胞骨架 MreB 结构，因此在细菌中发挥着两个重要功能。
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1
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Mol Microbiol. 2009 Apr;72(1):170-82. doi: 10.1111/j.1365-2958.2009.06632.x. Epub 2009 Feb 11.
2
Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix.通过将PBP1募集到MreB螺旋来调控枯草芽孢杆菌细胞壁形态发生
Mol Microbiol. 2009 Mar;71(5):1131-44. doi: 10.1111/j.1365-2958.2009.06601.x. Epub 2009 Jan 29.
3
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Proc Natl Acad Sci U S A. 2009 Jan 27;106(4):1239-44. doi: 10.1073/pnas.0810794106. Epub 2009 Jan 21.
4
RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E. coli.RodZ（YfgA）是大肠杆菌中MreB肌动蛋白细胞骨架正确组装和细胞形态所必需的。
EMBO J. 2009 Feb 4;28(3):193-204. doi: 10.1038/emboj.2008.264. Epub 2008 Dec 11.
5
Determination of bacterial rod shape by a novel cytoskeletal membrane protein.一种新型细胞骨架膜蛋白对细菌杆状形态的决定作用。
EMBO J. 2008 Dec 3;27(23):3081-91. doi: 10.1038/emboj.2008.234. Epub 2008 Nov 13.
6
Polymerization properties of the Thermotoga maritima actin MreB: roles of temperature, nucleotides, and ions.嗜热栖热放线菌肌动蛋白MreB的聚合特性：温度、核苷酸和离子的作用。
Biochemistry. 2008 Jan 15;47(2):826-35. doi: 10.1021/bi701538e. Epub 2007 Dec 21.
7
Cytoskeletal elements in bacteria.细菌中的细胞骨架成分。
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8
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Curr Biol. 2007 Feb 6;17(3):266-72. doi: 10.1016/j.cub.2006.11.069.
9
Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the function of MreB.动态定位以及与其他枯草芽孢杆菌肌动蛋白样蛋白的相互作用对MreB的功能很重要。
Mol Microbiol. 2006 Dec;62(5):1340-56. doi: 10.1111/j.1365-2958.2006.05457.x. Epub 2006 Oct 25.
10
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