Department of Biology and Biochemistry, University of Houston, Houston, TX 77204-5001, USA.
Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2544-9. doi: 10.1073/pnas.0906422107. Epub 2010 Jan 21.
The circadian clock controls many circadian outputs. Although a large number of transcripts are affected by the circadian oscillator, very little is known about their regulation and function. We show here that the Drosophila takeout gene, one of the output genes of the circadian oscillator, is regulated similarly to the circadian clock genes Clock (Clk) and cry. takeout RNA levels are at constant high levels in Clk(JRK) mutants. The circadian transcription factor PAR domain protein 1 (Pdp1epsilon) is a transcription factor that had previously been postulated to control clock output genes, particularly genes regulated similarly to Clk. In agreement with this, we show here that Pdp1epsilon is a regulator of takeout. Takeout levels are low in flies with reduced Pdp1epsilon and high in flies with increased amounts of Pdp1epsilon. Furthermore, flies with reduced or elevated Pdp1epsilon levels in the fat body display courtship defects, identifying Pdp1epsilon as an important transcriptional regulator in that tissue.
生物钟控制着许多生物钟输出。尽管大量的转录物受到生物钟振荡器的影响，但关于它们的调节和功能却知之甚少。我们在这里表明，果蝇的剔除基因（生物钟振荡器的输出基因之一）的调节方式与生物钟基因 Clock（Clk）和 cry 相似。在 Clk（JRK）突变体中，剔除 RNA 水平保持在较高水平。PAR 结构域蛋白 1（Pdp1epsilon）是一种先前被认为控制生物钟输出基因的转录因子，特别是那些与 Clk 调节相似的基因。与这一观点一致，我们在这里表明 Pdp1epsilon 是剔除的调节因子。剔除水平在 Pdp1epsilon 减少的果蝇中较低，在 Pdp1epsilon 增加的果蝇中较高。此外，在脂肪体中降低或升高 Pdp1epsilon 水平的果蝇表现出求爱缺陷，这表明 Pdp1epsilon 是该组织中重要的转录调节因子。
