Comparative Transcriptomic Analysis Reveals Molecular Profiles of Central Nervous System in Maternal Diapause Induction of .

Egg diapause in L. (Orthoptera: Acridoidea) is believed to be influenced by maternal photoperiod. However, the molecular mechanism regulating the phenomenon of maternal diapause induction is unclear. Here we performed transcriptomic analyses from the central nervous system (CNS) of migratory locusts under long and short photoperiods to identify differentially expressed genes (DEGs) related to diapause induction. There were total of 165750 unigenes from 569491 transcripts, and 610 DEGs were obtained in S_CNS (CNS of short photoperiod treated locusts) L_CNS (CNS of long photoperiod treated locusts). Of these, 360 were up-regulated, 250 were down-regulated, and 84 DEGs were found to be related to FOXO signaling pathways, including citrate cycle/TCA cycle, glycolysis/ gluconeogenesis, oxidative phosphorylation, and PI3K-Akt. The qRT-PCR validation of mRNA expression of 12 randomly selected DEGs showed consistency with transcriptome analysis. Furthermore, the gene thought to be involved in circadian rhythm was cloned and used for RNAi to observe its function in maternal diapause induction. We found that the mRNA level of was significantly lower in ds treatments as compared to the control under both long and short photoperiods. Similarly, the offspring diapause rate was significantly higher in ds treatment as compared to the control only in short photoperiod. This shows that the gene might be involved in the inhibition of maternal diapause induction of under short photoperiods. The present study provides extensive data of the CNS transcriptome and particular insights into the molecular mechanisms of maternal effects on egg diapause of As well for the future, the researchers can explore other factors and genes that may promote diapause in insect species.