Department of Biological Engineering, Seokyeong University, Seoul 136-704, Korea.
J Microbiol Biotechnol. 2010 Jan;20(1):94-100.
Zymomonas mobilis was immobilized in a modified graphite felt cathode with neutral red (NR-graphite cathode) and Saccharomyces cerevisiae was cultivated on a platinum plate anode to electrochemically activate ethanol fermentation. Electrochemical redox reaction was induced by 3 approximately 4 volt of electric potential charged to a cathode and an anode. Z. mobilis produced 1.3 approximately 1.5 M of ethanol in the cathode compartment and S. cerevisiae did 1.7 approximately 1.9 M in the anode compartment for 96 hr. The ethanol production by Z. mobilis immobilized in the NR-graphite cathode and S. cerevisiae cultivated on the platinum plate was 1.5 approximately 1.6 times higher than those cultivated in the conventional condition. The electrochemical oxidation potential greatly inhibited ethanol fermentation of Z. mobilis but did not S. cerevisiae. Total soluble protein pattern of Z. mobilis cultivated in the electrochemical oxidation condition was getting simplified in proportion to potential intensity based on SDS-PAGE pattern; however the SDS-PAGE pattern of protein extracted from S. cerevisiae cultivated in both oxidation and reduction condition was not changed. When Z. mobilis culture incubated in the cathode compartment for 24 hr was transferred to S. cerevisiae culture in the anode compartment, 0.8 approximately 0.9 M of ethanol was additionally produced by S. cerevisiae for another 24 hr. Conclusively, total 2.0 approximately 2.1 M of ethanol was produced by the electrochemical redox coupling of Z. mobilis and S. cerevisiae for 48 hr.
运动发酵单胞菌被固定在经过改良的石墨毡阴极上，并用中性红（NR-石墨阴极），而酿酒酵母则在铂板阳极上培养，以电化学激活乙醇发酵。电化学氧化还原反应是通过在阴极和阳极上施加 3 至 4 伏特的电势来诱导的。Z. mobilis 在阴极室中产生了 1.3 至 1.5 M 的乙醇，而 S. cerevisiae 在阳极室中产生了 1.7 至 1.9 M 的乙醇，持续了 96 小时。在 NR-石墨阴极中固定的 Z. mobilis 和在铂板上培养的 S. cerevisiae 的乙醇产量比在传统条件下培养的产量高 1.5 至 1.6 倍。电化学氧化电位极大地抑制了 Z. mobilis 的乙醇发酵，但对 S. cerevisiae 没有影响。基于 SDS-PAGE 模式，在电化学氧化条件下培养的 Z. mobilis 的总可溶性蛋白模式随着电势强度的增加而简化；然而，在氧化和还原条件下培养的 S. cerevisiae 提取的蛋白质的 SDS-PAGE 模式没有改变。当在阴极室中培养 24 小时的 Z. mobilis 培养物转移到阳极室中的 S. cerevisiae 培养物中时，S. cerevisiae 又产生了 0.8 至 0.9 M 的乙醇，持续了 24 小时。总之，通过 Z. mobilis 和 S. cerevisiae 的电化学氧化还原偶联，在 48 小时内总共产生了 2.0 至 2.1 M 的乙醇。
