College of Plant Protection, Shandong Agricultural University, Taian, Shandong, 271018, China.
J Microbiol Biotechnol. 2010 Jan;20(1):208-16.
Fusarium verticillioides is an important pathogen of maize responsible for ear rots, stalk rots and seeding blight worldwide. During the past decade F. verticillioides has caused several severe epidemics of maize seeding blight in many areas of china, which lead to significant losses. In order to understand molecular mechanisms regulating fungal development and pathogenicity in the pathogen, we isolated and characterized the gene fpk1 (GenBank accession NO. EF405959) encoding catalytic subunit of cAMP-dependent protein kinase, which include 1854 bp DNA sequence from ATG to TAA, with 1680 bp coding region, three intron (their length: 66bp, 54bp and 54bp), and the predicated protein had 559 aa. The mutantdeltafpk1, which was disrupted of fpk1 gene, showed reduced vegetative growth, fewer and shorter aerial mycelia, strongly impaired conidiation and reduced spore germination rate. After germinating, the fresh hypha was stubby and lack of branch. When inoculated in susceptible maize varieties, the infection of the mutantdeltafpk1 was delayed and the infection efficiency was reduced than that of the wild-type. All this indicated that the gene fpk1 participated in hyphal growth, conidiophore producing, spore germination and virulence in F. verticillioides.
串珠镰刀菌是一种重要的玉米病原菌，可引起玉米穗腐、茎腐和种子枯萎病，在全球范围内造成危害。在过去的十年中，串珠镰刀菌在中国许多地区引发了几次严重的玉米种子枯萎病流行，造成了重大损失。为了了解该病原菌中调节真菌发育和致病性的分子机制，我们分离并鉴定了编码 cAMP 依赖性蛋白激酶催化亚基的基因 fpk1（GenBank 登录号 EF405959），该基因包含从 ATG 到 TAA 的 1854 bp DNA 序列，包含 1680 bp 编码区、三个内含子（长度分别为 66bp、54bp 和 54bp），预测的蛋白质含有 559 个氨基酸。fpk1 基因缺失突变体 deltafpk1 的表型为生长缓慢、气生菌丝减少且变短、产孢能力显著下降、孢子萌发率降低。萌发后，新生菌丝粗短且缺乏分支。当接种在易感玉米品种上时，突变体 deltafpk1 的侵染延迟，侵染效率低于野生型。所有这些表明，基因 fpk1 参与了串珠镰刀菌的菌丝生长、分生孢子梗产生、孢子萌发和致病性。
