Pennington Caroline J, Edwards Dylan R
School of Biological Sciences, University of East Anglia, Norwich, UK.
Methods Mol Biol. 2010;622:159-73. doi: 10.1007/978-1-60327-299-5_9.
Quantitative reverse transcriptase polymerase chain reaction enables the accurate quantification of gene expression in cultured cells or small tissue samples. In this chapter, we describe the use of Taqman((R)) technology to measure expression of matrix metalloproteinases and related genes.
定量逆转录聚合酶链反应能够准确量化培养细胞或小组织样本中的基因表达。在本章中,我们描述了使用Taqman((R))技术来测量基质金属蛋白酶及相关基因的表达。