Kappelhoff Reinhild, Auf dem Keller Ulrich, Overall Christopher M
Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
Methods Mol Biol. 2010;622:175-93. doi: 10.1007/978-1-60327-299-5_10.
The degradome microarray - CLIP-CHIP - is a dedicated and focused array that allows the analysis of all proteases, non-proteolytic homologs, and protease inhibitor gene transcripts in the human and murine genomes at the mRNA transcript level.Based on unique 70-mer oligonucleotides, designed to match parts of the sequence of known or predicted protease and inhibitor mRNAs in both species and printed on a glass-matrix surface, the CLIP-CHIP microarray can be used to analyze differentially expressed protease and inhibitor gene products and give expression profiles for any analyzed sample.
降解组微阵列——CLIP-CHIP，是一种专门设计的聚焦阵列，可在mRNA转录水平分析人类和小鼠基因组中的所有蛋白酶、非蛋白水解同源物以及蛋白酶抑制剂基因转录本。基于独特的70聚体寡核苷酸，其设计用于匹配两个物种中已知或预测的蛋白酶和抑制剂mRNA序列的部分，并印刷在玻璃基质表面，CLIP-CHIP微阵列可用于分析差异表达的蛋白酶和抑制剂基因产物，并给出任何分析样本的表达谱。
