金属蛋白酶及其抑制剂的原位杂交
In situ hybridization for metalloproteinases and their inhibitors.
作者信息
Hurskainen Tiina L, Apte Suneel S
机构信息
Clinical Research Center, Oulu University Hospital, Oulu, Finland.
出版信息
Methods Mol Biol. 2010;622:195-209. doi: 10.1007/978-1-60327-299-5_11.
PMID:20135283
Abstract
In situ hybridization (ISH) is an invaluable tool in understanding tissue-specific gene expression and gene regulation within a spatial context and at a resolution that is not possible by any other method. In this chapter, we provide ISH methodology that has successfully been applied to the detection of metalloproteinases and their inhibitors.
摘要
原位杂交（ISH）是一种非常宝贵的工具，可用于在空间背景下以及以其他任何方法都无法实现的分辨率来理解组织特异性基因表达和基因调控。在本章中，我们提供了已成功应用于检测金属蛋白酶及其抑制剂的原位杂交方法。
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