Laxton Ross, Ye Shu
Barts and the London School of Medicine and Dentistry, William Harvey Research Institute, London, UK.
Methods Mol Biol. 2010;622:221-9. doi: 10.1007/978-1-60327-299-5_13.
Many molecular genetic studies of human diseases involve determining the genotypes for single nucleotide polymorphisms. This chapter summarises a number of different techniques for the single nucleotide polymorphism genotyping which can be applied to MMP genes. The chapter also provides a protocol and technical notes for the 5'-nuclease assay, one of the most commonly used genotyping techniques.
许多人类疾病的分子遗传学研究都涉及确定单核苷酸多态性的基因型。本章总结了多种可应用于MMP基因的单核苷酸多态性基因分型的不同技术。本章还提供了最常用的基因分型技术之一——5'-核酸酶测定法的实验方案和技术说明。
