采用对溴四咪唑抑制法测定血浆中肠源性碱性磷酸酶
Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition.
作者信息
Kuwana T, Rosalki S B
机构信息
Department of Chemical Pathology and Human Metabolism, Royal Free Hospital, London.
出版信息
J Clin Pathol. 1991 Mar;44(3):236-7. doi: 10.1136/jcp.44.3.236.
L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If carried out in parallel with wheat-germ lectin precipitation of bone ALP, subtraction of intestinal ALP activity from that of non-bone ALP in the supernatant can be used to measure the ALP that originates from the liver in men and non-pregnant women.
L-对溴四咪唑用于抑制血浆中（肝脏或骨骼来源的）非肠道碱性磷酸酶（EC 3.1.3.1；ALP），通过未受抑制的活性来测定肠道碱性磷酸酶。该测定方法简便，且与免疫捕获测定法的测量结果相关性良好。若与小麦胚芽凝集素沉淀法并行检测骨骼碱性磷酸酶，可通过从上清液中非骨骼碱性磷酸酶活性中减去肠道碱性磷酸酶活性，来测定男性和非孕女性肝脏来源的碱性磷酸酶。
Zotero 插件