Zhang Yao, Chen Qi, Fang Ning
Department of Hematology, The Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Feb;18(1):195-8.
This study was aimed to detect the CD3(+) count, ratio of CD3(+)/CD30(+) T-lymphocytes, the apoptosis rate of activated T-lymphocytes in peripheral blood mononuclear cells and the circulating platelet counts in patients with chronic idiopathic thrombocytopenic purpura (ITP), and to investigate the relationship between them. 40 cases of chronic ITP patients were taken as the research group and 24 healthy persons were served as the control group. The CD3 count, ratio of CD3/CD30 T-lymphocytes and the apoptosis rate of activated T-lymphocytes were detected by flow cytometry, the platelet count was measured at the same time. The difference between 2 groups and correlation of the activated T lymphocyte CD30 expression rate with apoptotic rate and platelet count in patients were analysed. The results showed that there was no significant difference in the count of peripheral blood CD3(+) cell between the research group and the control group (p > 0.05). The expression of CD30 on activated T-lymphocytes in ITP group was obviously higher than that in control group (p < 0.01). The apoptosis rate of activated T-lymphocyte in ITP group was remarkably lower than that in control group (p < 0.01). The expression of CD30 on activated T-lymphocyte exhibited a negative correlation with the apoptosis rate (r = -0.786, p < 0.01). The apoptosis rate of activated T-lymphocyte was positively correlated with the platelet count of peripheral blood (r = 0.680, p < 0.01). It is concluded that the high expression of CD30 on activated T-lymphocytes in patients with chronic ITP results in the inhibition of apoptosis, which leads to immune disorder and thrombocytopenia.
本研究旨在检测慢性特发性血小板减少性紫癜（ITP）患者外周血单个核细胞中CD3(+)计数、CD3(+)/CD30(+) T淋巴细胞比例、活化T淋巴细胞凋亡率及循环血小板计数，并探讨它们之间的关系。选取40例慢性ITP患者作为研究组，24例健康人作为对照组。采用流式细胞术检测CD3计数、CD3/CD30 T淋巴细胞比例及活化T淋巴细胞凋亡率，同时检测血小板计数。分析两组之间的差异以及患者活化T淋巴细胞CD30表达率与凋亡率和血小板计数的相关性。结果显示，研究组与对照组外周血CD3(+)细胞计数差异无统计学意义（p>0.05）。ITP组活化T淋巴细胞上CD30的表达明显高于对照组（p<0.01）。ITP组活化T淋巴细胞凋亡率明显低于对照组（p<0.01）。活化T淋巴细胞上CD30的表达与凋亡率呈负相关（r=-0.786，p<0.01）。活化T淋巴细胞凋亡率与外周血血小板计数呈正相关（r=0.680，p<0.01）。结论：慢性ITP患者活化T淋巴细胞上CD30的高表达导致凋亡受抑制，进而引起免疫紊乱和血小板减少。
