Wu Xiao-Jin, He Jun, Wu De-Pei, Sun Ai-Ning, Bao Xiao-Jing, Qiu Qiao-Cheng, Yuan Xiao-Ni, Li Wei-Yang, Chang Hui-Rong, Han Yue, Ma Xiao, Zhu Zi-Ling, Xu Yang, Feng Yu-Feng, Dai Li-Jun
The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
Zhonghua Xue Ye Xue Za Zhi. 2009 Nov;30(11):741-4.
To explore the relationship between CMV reactivation and KIR haplotype or HLA-Cw genotype in patients after unrelated-donor hematopoietic stem cell transplantation (HSCT).
From January 2003 to December 2008 the HLA-Cw/KIR genotype of 48 patient-donor pairs were determined by polymerase chain reaction with sequence specific primers (PCR-SSP) and sequence specific nucleotide (PCR-SSOP). Posttransplant CMV reactivation was performed by immune histochemically assay.
Of 48 patients, 15 were transplanted from unrelated donors with an antigen mismatch for HLA Cw and 33 patient-donor pairs were matched for HLA-Cw. The CMV reaction rate was 66.7% for HLA-Cw mismatch group and 48.5% for HLA-Cw match group (chi(2) = 1.39, P = 0.2375). Thirty-seven donor-patients pairs belonged to group C1 and 11 to group C2, and CMV reaction rate was 64.9% in group C1 and 18.2% in group C2 (chi(2) = 18.13, P < 0.0001). Twenty-six patients received graft from KIR haplotype A (group A donor) and 22 from KIR haplotype B donors (group B donor) and CMV reaction rate was 57.7% in group A donor and 50.0% in group B donor (chi(2) = 0.28, P = 0.5941). The number of donor activating KIRs (aKIRs) was less than that of recipient aKIRs in 34 patient-donor pairs in which the CMV reaction rate was 70.6%, and the number of donor aKIRs was more than that of recipient aKIRs in 14 patient-donor pairs in which the CMV reactivation was 14.3%. There was a significan difference between the two group (chi(2) = 12.44, P = 0.0004).
KIR and HLA-Cw genotypes influence the rate of CMV reactivation following non-T cell deleted unrelated donor hematopoietic cell transplantation.
探讨非亲缘供者造血干细胞移植（HSCT）后患者巨细胞病毒（CMV）再激活与杀伤细胞免疫球蛋白样受体（KIR）单倍型或人类白细胞抗原Cw（HLA-Cw）基因型之间的关系。
2003年1月至2008年12月，采用序列特异性引物聚合酶链反应（PCR-SSP）和序列特异性核苷酸（PCR-SSOP）方法，对48例患者-供者对的HLA-Cw/KIR基因型进行检测。移植后CMV再激活情况采用免疫组织化学方法检测。
48例患者中，15例接受了HLA Cw抗原不匹配的非亲缘供者移植，33例患者-供者对HLA-Cw匹配。HLA-Cw不匹配组CMV反应率为66.7%，HLA-Cw匹配组为48.5%（χ² = 1.39，P = 0.2375）。37例供者-患者对属于C1组，11例属于C2组，C1组CMV反应率为64.9%，C2组为18.2%（χ² = 18.13，P < 0.0001）。26例患者接受了KIR单倍型A供者的移植物（A组供者），22例接受了KIR单倍型B供者的移植物（B组供者），A组供者CMV反应率为57.7%，B组供者为50.0%（χ² = 0.28，P = 0.5941）。在34例患者-供者对中，供者激活型KIR（aKIR）数量少于受者，其CMV反应率为70.6%；在14例患者-供者对中，供者aKIR数量多于受者，其CMV再激活率为14.3%。两组间差异有统计学意义（χ² = 12.44，P = 0.0004）。
KIR和HLA-Cw基因型影响非T细胞去除的非亲缘供者造血细胞移植后CMV再激活率。
