Wu Xiao-Jin, He Jun, Wu De-Pei, Bao Xiao-Jing, Xu Chao, Zhou Hui-Fen, Sun Ai-Ning, Han Yue, Tang Xiao-Wen, Fu Zheng-Zheng, Ma Xiao
Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
Zhonghua Nei Ke Za Zhi. 2013 Feb;52(2):161-5.
To explore the influence of the killer cell immunoglobulin like receptor (KIR) gene polymorphism on cytomegalovirus (CMV) infection and pathogenesis after hematopoietic stem cell transplantation (HSCT).
The KIR genotype was determined by sequence-specific primer polymerase chain reaction (PCR-SSP) in 138 pairs of donors and recipients before HSCT during October, 2005 and May, 2011. Posttransplant monitoring for CMVpp65 antigen was performed by indirect immune histochemically assays since week 2 after transplantation. The differences between CMV positive group and negative group, inhibitive and active KIR of donors and recipients, and KIR haplotype frequency of donors and recipients were analyzed.
There were no significant differences in frequency of KIR gene and haplotype AA, AB, BB between the donors and recipients. The frequencies of 2DS2 and 2DS4 * 003-007 of donors in CMV positive group were obviously lower than those in CMV negative group with significant differences (8% vs 16% , P = 0.0420; 3% vs 13%, P = 0.0050). There was no significant difference in KIR gene between CMV positive group and CMV negative group. The CMV infection rates of haplotype AA, BB, AB donors were 64.38%, 36.84% and 50.00%, while CMV infection rates of haplotype AA, BB, AB recipients were 53.73%, 46.15% and 51.72%, respectively. The CMV infection rate was higher in the patients received KIR haplotype AA donor than in those received KIR haplotype BB donor (36.84% vs 64.38%, P = 0.0299). 2DS4 x 003-007 and haplotype BB of donor were found associated with CMV infection in multifactor analysis.
KIR genotypes of donors are associated with CMV infection after HSCT.
探讨杀伤细胞免疫球蛋白样受体（KIR）基因多态性对造血干细胞移植（HSCT）后巨细胞病毒（CMV）感染及发病机制的影响。
2005年10月至2011年5月期间，采用序列特异性引物聚合酶链反应（PCR-SSP）对138对HSCT供受者在移植前进行KIR基因分型。自移植后第2周起，通过间接免疫组织化学法对CMV pp65抗原进行移植后监测。分析CMV阳性组与阴性组之间、供受者抑制性和活性KIR以及供受者KIR单倍型频率的差异。
供受者之间KIR基因及单倍型AA、AB、BB的频率无显著差异。CMV阳性组供者中2DS2和2DS4 * 003 - 007的频率明显低于CMV阴性组，差异有统计学意义（8%对16%，P = 0.0420；3%对13%，P = 0.0050）。CMV阳性组与CMV阴性组之间KIR基因无显著差异。单倍型AA、BB、AB供者的CMV感染率分别为64.38%、36.84%和50.00%，而单倍型AA、BB、AB受者的CMV感染率分别为53.73%、46.15%和51.72%。接受KIR单倍型AA供者的患者CMV感染率高于接受KIR单倍型BB供者的患者（36.84%对64.38%，P = 0.0299）。多因素分析发现供者的2DS4 x 003 - 007和单倍型BB与CMV感染有关。
供者的KIR基因分型与HSCT后CMV感染有关。
