Huang Fang, Qu Mei, Liu Yuan, Yan Han-qiu, Gao Zhi-yong, Dou Xiang-feng, Zhang Hai-yan, Zhang Zhen, Ma Jian-xin, Guo Jing
Department of Infectious and Endemic Diseases Control, Beijing Center for Disease Prevention and Control, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2009 Sep;43(9):789-92.
To understand pathogen patterns of enteric infectious diseases and its impact on this pattern due to aggregation of a great deal of foreign visitors during Beijing Olympic Games.
The diarrheal patient's rectal swabs and stool specimens were collected from Olympic stadium and hospitals of four districts, including Dongcheng, Xicheng, Haidian and Chaoyang. Enteric multiple pathogens were detected from the total 45 specimens. The culture method was used for the enteric bacteria, ELISA and RT-PCR for the enteric viruses. Molecular typing of Salmonella Enteritidis isolation was completed by PFGE.
It was found that 26 out of 45 cases were positive with 57.8 percent for pathogen detection, and 24 were identified as enteric pathogenic bacteria, including Vibrio parahaemolyticus, Salmonella, diarrheagenic Escherichia coli and Campylobacter jejuni, two as norovirus. There were mixed infections of two pathogenic bacteria for three cases. Ten kinds of pathogens were detected from foreign cases, while five kinds from Chinese cases. A total of 5 PFGE patterns were identified in 10 Salmonella Enteritidis isolates from national and foreign diarrheal cases, which were concentrative in some extent.
Vibrio parahaemolyticus, Salmonella, diarrheagenic Escherichia coli and Campylobacter jejuni were found to be the primary bacterial pathogens during the Olympic Games. Enteric virus infection existed in summer diarrhea.
了解肠道传染病病原体谱以及北京奥运会期间大量外国游客聚集对其谱型的影响。
从奥林匹克体育场馆及东城区、西城区、海淀区和朝阳区4个区的医院收集腹泻患者的直肠拭子和粪便标本。从总共45份标本中检测肠道多种病原体。肠道细菌采用培养法检测，肠道病毒采用酶联免疫吸附测定法（ELISA）和逆转录-聚合酶链反应（RT-PCR）检测。肠炎沙门菌分离株通过脉冲场凝胶电泳（PFGE）进行分子分型。
45例中26例病原体检测阳性，阳性率为57.8%，其中24例鉴定为肠道病原菌，包括副溶血性弧菌、沙门菌、致泻性大肠埃希菌和空肠弯曲菌，2例为诺如病毒。3例为两种病原菌混合感染。外国病例检测出10种病原体，中国病例检测出5种。从国内外腹泻病例的10株肠炎沙门菌分离株中鉴定出5种PFGE图谱，图谱在一定程度上具有集中性。
副溶血性弧菌、沙门菌、致泻性大肠埃希菌和空肠弯曲菌是奥运会期间主要的细菌性病原体。肠道病毒感染存在于夏季腹泻中。
