[锰暴露工人的脑磁共振成像变化]

[Variations of brain magnetic resonance imaging among manganese-exposed workers].

作者信息

Qin Wei-ping, Fu Xue, Jiang Yue-ming, Long Li-ling, Li Xiang-rong, Chen Hai-bin, Huang Zhi-bi, Zhao Wei-jia, Mo Xue-an, Zheng Wei

机构信息

Guangxi Institute for Occupational Disease Prevention and Treatment, Nanning, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2009 Sep;43(9):793-7.

PMID:20137563

Abstract

OBJECTIVE

Variations of the signal intensities in the magnetic resonance (MR) T(1)-weighted image (T(1)WI) of globus pallidus among manganese(Mn)-exposed workers were explored to provide a scientific basis for exposed biomarker of manganese-injured central nervous system (CNS).

METHODS

The brain MR T(1) and T(2) WI in eighteen male asymptomatic Mn-exposed, eight manganism and nine healthy control workers were examined routinely by adopting a 1.5 Tesla signal superconducting system. The SIGP and the signal intensity in frontal white matter (SIFWM) in the same side were determined, then pallidal index (PI) was calculated. Concentration of MnO(2) in workplaces and content of manganese in red blood cell (MnRBC) among workers were respectively determined by flame atomic absorption spectrometer (AAS) and inductively coupled plasma-atomic emission spectrophotometry (ICP-AES). The follow-up investigation in the eight high Mn-exposed workers was made one year later.

RESULTS

The results showed that the median of air MnO(2) in smelting workplace was 0.64 mg/m(3)(0.07 - 5.40 mg/m(3)), which were respective 0.56 mg/m(3)(0.09 - 1.71 mg/m(3)) in power distribution room (low Mn-exposure) and 0.89 mg/m(3) (0.07 - 5.40 mg/m(3)) in furnace (high Mn-exposure). PI in the Mn-exposed and high Mn-exposed workers were both higher than those of the manganism and control workers(116.4 +/- 8.2, 119.0 +/- 7.9, 105.3 +/- 8.4 and 102.2 +/- 1.5, respectively. Mn vs control, t' = 7.146, P = 0.000; Mn vs manganism, t = 3.181, P = 0.004. High Mn-exposure vs control, t' = 7.446, P = 0.000; high Mn-exposure vs manganism, t = 3.763, P = 0.001). The increased signal in T(1)WI of globus pallidus was observed in Mn-exposed workers, especially in high Mn-exposed workers. The content of manganese in red blood cell of Mn-exposed and control workers was significantly higher than those of the manganism workers [(151.6 +/- 40.5) ng/ml, (149.2 +/- 21.3) ng/ml, (154.5 +/- 46.6) ng/ml, (144.4 +/- 14.2) ng/ml, (20.8 +/- 7.4) ng/ml respectively. The difference was significant in statistics. Manganism vs control, t = 20.206, P = 0.000; manganism vs Mn, t' = 13.144, P = 0.000; manganism vs low and high Mn, t' = 12.964, 9.957, respectively, P = 0.000]. Only a decreased median of air MnO(2) in furnace was found one year later (0.89, 0.31 mg/m(3), Z = -2.142, P = 0.032). The difference was significant in statistics.

CONCLUSION

Our data suggests that SIGP of MR T(1)WI among workers was obviously increased by manganese-exposure. PI may be taken as the signal of CNS injury which was induced by manganese-exposure.

摘要

目的

探讨锰暴露工人苍白球磁共振（MR）T1加权像（T1WI）信号强度变化，为锰致中枢神经系统（CNS）损伤的暴露生物标志物提供科学依据。

方法

采用1.5特斯拉超导系统对18名男性无症状锰暴露工人、8名锰中毒工人和9名健康对照工人进行脑部MR T1WI和T2WI常规检查。测定苍白球信号强度比（SIGP）和同侧额叶白质信号强度（SIFWM），计算苍白球指数（PI）。分别采用火焰原子吸收光谱仪（AAS）和电感耦合等离子体原子发射光谱法（ICP-AES）测定工作场所二氧化锰浓度和工人红细胞锰含量。对8名高锰暴露工人进行1年后的随访调查。

结果

结果显示，冶炼车间空气中二氧化锰中位数为0.64mg/m³（0.07～5.40mg/m³），配电室（低锰暴露）为0.56mg/m³（0.09～1.71mg/m³），熔炉（高锰暴露）为0.89mg/m³（0.07～5.40mg/m³）。锰暴露工人和高锰暴露工人的PI均高于锰中毒工人和对照工人（分别为116.4±8.2、119.0±7.9、105.3±8.4和102.2±1.5。锰暴露组与对照组比较，t' = 7.146，P = 0.000；锰暴露组与锰中毒组比较，t = 3.181，P = 0.004。高锰暴露组与对照组比较，t' = 7.446，P = 0.000；高锰暴露组与锰中毒组比较，t = 3.763，P = 0.001）。锰暴露工人，尤其是高锰暴露工人的苍白球T1WI信号增强。锰暴露工人和对照工人的红细胞锰含量均显著高于锰中毒工人[分别为（151.6±40.5）ng/ml、（149.2±21.3）ng/ml、（154.5±46.6）ng/ml、（144.4±14.2）ng/ml、（20.8±7.4）ng/ml。差异有统计学意义。锰中毒组与对照组比较，t = 20.206，P = 0.000；锰中毒组与锰暴露组比较，t' = 13.144，P = 0.000；锰中毒组与低锰和高锰暴露组比较，t'分别为12.964、9.957，P = √0.000]。1年后仅发现熔炉空气中二氧化锰中位数下降（0.89，0.31mg/m³，Z = -2.142，P = 0.032）。差异有统计学意义。