Lin Hong-fei, Zhu Zhi-rui, Hu Zhi-yong
Department of Anesthesiology, Children's Hospital of Zhejiang University School of Medicine, Hangzhou 310003, China.
Zhonghua Yi Xue Za Zhi. 2009 Nov 10;89(41):2943-5.
To investigate if sevoflurane preconditioning attenuate neuronal apoptosis induced by ischemia-reperfusion.
Thirty-six male SD rats weighing 250 - 300 g were randomly divided into three groups (n = 12 each): control group (group C), ischemia-reperfusion group (group IR) (rats were established cerebral artery clamped and reperfusion model), sevoflurane preconditioning group (group S) (rats were established cerebral artery clamped and reperfusion model after 1 h 2.4% sevoflurane preconditioning). Apoptosis neurons were observed by Hematoxylin and Eosin (HE) staining and transmission electron microscope, TdT mediated Dutp nick end labeling (TUNEL) method was used to count apoptosis neurons density, fresh ischemic brain tissue was taken out, while Caspase-3 zymogen and 20 000 segment were checked by Western blot.
apoptosis neurons in group IR were more than ones in group S under HE staining and light microscope and transmission electron microscope, and apoptosis neurons density (cell number/0.1 mm(2)) by TUNEL staining: group C, 13.0 +/- 1.4; group IR, 189.8 +/- 6.8; group S, 110.5 +/- 4.3, the relative gray values of the contents of procaspase-3 and its 20 000 cleavage fragment were 16.72 +/- 3.0, 76.1 +/- 3.4, 51.2 +/- 3.1 and 8.2 +/- 2.3, 59.0 +/- 6.3, 31.2 +/- 5.4 respectively.
Sevoflurane pretreatment can protect neuron on ischemia-reperfusion injury by attenuating neuronal apoptosis in rats.
探讨七氟醚预处理是否能减轻缺血再灌注诱导的神经元凋亡。
将36只体重250 - 300 g的雄性SD大鼠随机分为三组（每组n = 12）：对照组（C组）、缺血再灌注组（IR组）（大鼠建立脑动脉夹闭再灌注模型）、七氟醚预处理组（S组）（大鼠在2.4%七氟醚预处理1小时后建立脑动脉夹闭再灌注模型）。通过苏木精-伊红（HE）染色、透射电子显微镜观察凋亡神经元，采用TdT介导的dUTP缺口末端标记（TUNEL）法计数凋亡神经元密度，取新鲜缺血脑组织，采用蛋白质免疫印迹法检测Caspase-3酶原及其20 000片段。
在HE染色、光学显微镜和透射电子显微镜下，IR组凋亡神经元多于S组，TUNEL染色凋亡神经元密度（细胞数/0.1 mm²）：C组为13.0±1.4；IR组为189.8±6.8；S组为110.5±4.3，procaspase-3及其20 000裂解片段含量的相对灰度值分别为16.72±3.0、76.1±3.4、51.2±3.1和8.2±2.3、59.0±6.3、31.2±5.4。
七氟醚预处理可通过减轻大鼠神经元凋亡对缺血再灌注损伤起到神经元保护作用。
