[呼吸道合胞病毒对人胚肺成纤维细胞细胞间黏附分子-1 mRNA及蛋白表达的影响]

[Effects of respiratory syncytial virus upon expressions of mRNA and protein of intercellular adhesion molecule-1 in human embryonic lung fibroblast cells].

作者信息

Chen Cai-xia, Wang Shou-chuan, Hu Yu, Xu Jian-ya

机构信息

Children's Medical Center, Second Hospital Affiliated to Nanjing Medical University, Nanjing 210011, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2009 Sep 15;89(34):2420-3.

PMID:20137698

Abstract

OBJECTIVE

To explore the effects of respiratory syncytial virus (RSV) upon the expressions of mRNA and protein of intercellular adhesion molecule-1 (ICAM-1) in human embryonic lung fibroblast cells.

METHODS

The expressions of mRNA and protein of ICAM-1 were determined in human embryonic lung fibroblast cells after being infected by the LONG strain type A RSV and in normal fibroblast cells with real-time PCR and flow cytometry.

RESULTS

The mRNA of ICAM-1 expression in human embryonic lung fibroblast cells was 2.51 times at 24 h post-infection as that in normal fibroblast cells (P < 0.05). The protein of ICAM-1 expression of RSV control group (1.25 +/- 0.09, 1.87 +/- 0.18, 4.78 +/- 0.52, 13.34 +/- 0.64, 1.58 +/- 0.37) were significantly higher than those of normal cell group (0.21 +/- 0.06, 0.30 +/- 0.06, 0.29 +/- 0.07, 0.35 +/- 0.17, 0.35 +/- 0.14) at 12, 24, 48, 72 and 96 h post-infection (all P < 0.01). The protein of ICAM-1 expression of RSV control group achieved a peak value between 48 h and 72 h, and then it decreased significantly at 96 h.

CONCLUSION

Lung fibroblast cell and ICAM-1 may play some roles in pathogenic mechanism of RSV viral pneumonia.

摘要

目的

探讨呼吸道合胞病毒（RSV）对人胚肺成纤维细胞细胞间黏附分子-1（ICAM-1）mRNA及蛋白表达的影响。

方法

采用实时荧光定量PCR及流式细胞术检测人胚肺成纤维细胞感染A 型RSV长株后及正常成纤维细胞中ICAM-1 mRNA及蛋白的表达。

结果

感染后24 h，人胚肺成纤维细胞中ICAM-1 mRNA表达量为正常成纤维细胞的2.51倍（P<0.05）。感染后12、24、48、72及96 h，RSV感染组ICAM-1蛋白表达量（1.25±0.09、1.87±0.18、4.78±0.52、13.34±0.64、1.58±0.37）均显著高于正常细胞组（0.21±0.06、0.30±0.06、0.29±0.07、0.35±0.17、0.35±0.14）（均P<0.01）。RSV感染组ICAM-1蛋白表达量在48~72 h达到峰值，96 h时显著下降。